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商品編號


TTP-200



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Protease



Concentration

2mg/ml


Size

2 mg


商品信息

TurboTEV (Tobacco etch virus) Protease is a highly enhanced site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between the Gln and Gly. TurboTEV protease is resistant to auto-inactivation under normal reaction conditions and works as a better catalyst than the wild-type enzyme. It is a very useful reagent for cleaving fusion proteins due to its high specificity and high activity rate without the requirements of specialized buffer.





Description:
TurboTEV (Tobacco Etch Virus) Protease is a highly enhanced site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between the Gln and Gly. TurboTEV protease is resistant to auto-inactivation under normal reaction conditions and works as a better catalyst than the wild-type enzyme. It is a very useful enzyme for cleaving fusion proteins due to its high specificity and high activity rate without the requirements of specialized buffer. It has both a His-tag and GST tag, which allow it to be removed by Ni-chelating or GSH resin.
Application:
- Removal of fusion tags from recombinant proteins
- Highly dynamic and precise cleavage cap
ABI
lities
- Purification of proteins and peptides
Source:
E. coli
derived from Tobacco Etch Virus.
Supplied With:
TEV substrate, 50 ?l
10X TEV buffer
Label:
His-tag and GST tag
Reaction Conditions:
Turbo TEV protease is maximally active at 34°C, but its recommended to perform digests at room temperature (20°C) or 4°C. The activity of TEV protease is approximately 3-fold greater at 20 °C than at 4°C.
10X TEV buffer
500 mM Tris-HCl, 1.5 M NaCl, pH 8.0


Recommended Storage Condition:
-20°C


Figures:
Figure 1
Figure 2. 69.8 kDa TEV substrate protein is incubated with TurboTEV. The cleaved products are 41.2 kDa and 27.7 kDa, respectively.