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商品編號


N0103B



品牌


Accelagen



公司


Accelagen



公司分類(lèi)


Enzyme Toolbox



Concentration

250 Units/?l


Size

Bulk

商品信息


Description:
TurboNuclease
TM
is an ultra-pure recombinant form of
Serratia marcescens
extracellular endonuclease (encoded by the same gene as Benzonase) produced in
E. coli
using a proprietary process. This non-specific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is free of protease activity. TurboNuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.

Protein Type:
Endonuclease

Source:

E. coli

Unit Definition:
One unit of TurboNuclease converts 1.0 OD
260
of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37
o
C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl
2
. This corresponds to complete digestion of 50 ?g of salmon sperm DNA into oligonucleotides. TurboNuclease has similar specific activity as Benzonase

Storage:
Store TurboNuclease at -20
o
C.

Endotoxin Level:
< 0.1 EU / 1,000 units of TurboNuclease as determined by the Endo-Safe LAL Assay.