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商品編號


P0111B



品牌


Accelagen



公司


Accelagen



公司分類(lèi)


Enzyme Toolbox



Concentration

400,000 units/ml


Size

Bulk

商品信息


Description:
Lam
BD
a protein phosphatase (&lam
BD
a;PPase) is a Mn
2+
dependent protein phosphatase with activity to remove phosphate groups from serine, threonine, tyrosine, and histidine residues. It is the 221 amino-acid product of the ORF221 open re
ADI
ng frame of bacteriophage lam
BD
a (1). Recombinant &lam
BD
a;PPase is purified from a strain of E. coli that carries the &lam
BD
a;PPase gene. &lam
BD
a;PPase has minimum 95% purity with a molecular weight of 25 kDa as determined by SDS-PAGE and Coomassie Blue staining. It has no detectable protease and nuclease activity.

Protein Type:
Peotein phosphatase

Source:
E. coli

Specific Activity:
>600,000 units/mg
1 unit of &lam
BD
a;PPase hydrolyzes 1 nmol of
p
-nitrophenyl phosphate per minute at 30°C in assay buffer of 50 mM HEPES pH 7.5, 100 mM NaCl. 2 mM DTT, and 1 mM MnCl
2
.

Storage/Handling:
Store at below -70
o
C. Shipped with dry ice.