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商品編號


R3142S-20,000 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


Restriction Endonucleases



Concentration

20,000 units/ml


Size

20,000 units


商品信息

Description:
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High Fidelity (HF?) Restriction Enzymes have 100% activity in CutSmart? Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.HF enzymes also exhibit dramatically reduced star activity.HF enzymes are all Time-Saver? qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA.Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
NEB
extensively performs quality controls on all standard and high-fidelity(HF?) restriction enzymes. Examples of nuclease contamination studies for someof our HF restriction enzymes are shown below.
Restriction Enzyme Competitor Study: Nuclease Contamination
EcoRI, NotI, and BamHI from multiple suppliers were tested in reactionscontaining a fluorescent labeled single stranded, double stranded blunt,3’overhang or 5’ overhang containing oligonucleotides. The percent degradationis determined by capillary electrophoresis and peak analysis. The resolution isat the single nucleotide level.
Product Source
An
?E. coli
strain that carries the cloned and modified KpnI gene from?
Klebsiella pneumoniae
?OK8 (
ATCC
49790)
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Gel Lo
ADI
ng Dye, Purple (6X)
25
6X
Notes:
Acc65I is an isoschizomer of KpnI. KpnI produces a 4-base 3? extension, whereas Acc?65I produces a 4-base 5? extension.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch? PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.