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商品編號


M0290S-5,000 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


DNA Modifying Enzymes



Concentration

10,000 units/ml


Size

5,000 units

商品信息

Description:
Alkaline Phosphatase, CalfIntestinal (CIP) nonspecifically catalyzes thedephosphorylation of 5? and 3? ends of DNAand RNA phosphomonoesters. Also, CIPhydrolyses ribo-, as well as deoxyribonucleosidetriphosphates (NTPs and dNTPs). CIP is useful inmany molecular
BIOLOG
y applications such as theremoval of phosphorylated ends of DNA and RNAfor subsequent use in cloning or end-labeling ofprobes. In cloning, dephosphorylation preventsreligation of linearized plasmid DNA. The enzymeacts on 5? protruding, 5? recessed and blunt ends.CIP may also be used to degrade unincorporateddNTPs in PCR reactions to prepare templates forDNA sequencing or SNP analysis.
Product Source
Calf intestinal mucosa
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
CIP, as are most alkaline phosphatases, isa Zn2+ and Mg2+-dependent enzyme. Ourformulation of its storage buffer provides Zn2+and Mg2+, which does not require supplementalzinc or other additives in reactions with CIP.CIP is also active in 1X
NEB
uffers 1.1, 2.1,3.1, as well as
NEB
uffers 1, 2, 3, 4 and unique
NEB
uffer for EcoRI.CIP activity is enhanced in the presence ofmonovalent salts.CIP is inhibited by metal chelators (e.g. EDTA),inorganic phosphate and phosphate analogs.CIP activity is decreased in the presence of reducing agents (DTT, β-ME).