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商品編號


LS006322



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分類(lèi)


Products



Size

100 ku

商品信息
DNase I, Recombinant, Animal Free Bioprocess GradeDNase I, Recombinant, Produced in Pichia pastoris, Animal Origin Free, Bioprocess GradeChromatographically purified AOF bioprocessing grade. Supplied as > 2,000 units per lyophilized powder containing glycine mg dry weight as a st
ABI
lizer. For the removal of DNA in bioprocessing and primary stem cell isolation application. May contain protease and RNase. Store at 2-8°C. PROTECT FROM MOISTURE.Store at 2-8°C.≥2,000 Kunitz units per mg dry weight
Source:
Pichia pastoris
I.U.B.: 3.1.21.1
?
Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast,
Pichia

pastoris,
to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.

Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.

Recombinant DNase I is suitable for such applications as:
? Removing genomic DNA from RNA preparations prior to RT-PCR
? Degradation of DNA templates after transcription reactions
? Removing unwanted DNA from samples prior to Northern blotting
? Removing DNA during biopharma and bioprocessing procedures

Unit Definition:
One Unit causes an increase in absorbance at 260nm of 0.001 per minute at 25
o
C when acting upon highly polymerized DNA at pH 5.0, which is the same as other
Worthington
DNase I products.

Note:
Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at
Worthington
. As measured at
Worthington
, One Kunitz unit digests 1
m
g of calf thymus (or pUC19 or
l
-phage) DNA in 10 minutes at 37
o
C in 50mM Tris, 1mM Mg
2+
, 1mM Ca
2+
, pH 7.8. Correlation of digestion units with Kunitz units may be different in other buffer systems.

Storage Buffer (DR1S):
5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.

DNase I Reaction Buffer (10X):
500mM Tris-HCl, 10mM MgSO
4
, 1mM CaCl
2
, pH 7.8, provided.
Related Products:
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E?RASE RNase A/T1 Blend (RCT)
Histones (H, NHL)
Lysozyme (LY/LYSF)?
Nuclease, Micrococcal (NFCP)
Nuclease, S1 (SINUC/SINUCL)
Nucleic Acids, DNA, E.coli, Lam
BD
a/Fragments,RNA
Phosphatase, Alkaline (CAP/BAPF/BAPC/BAPSF/PC)
Phosphodiesterase I (VPH)?
Phosphodiesterase II (SPH)?
Proteinase K (PROK/PROKS)
Reverse Transcriptase, Recombinant HIV (RTHIV)?
Ribonuclease A (R/RAF/RASE/RS/RPDF)
Ribonuclease T1, Animal Origin Free (RT1S)
?