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商品編號


LS005410



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分類(lèi)


Products



Size

20 ku

商品信息
Deoxyribonuclease II, PurifiedChromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966). A dialyzed, lyophilized powder.Store at -20°C.REQUIRES SPECIAL SHIPPING: ICE PACK≥12,000 units per mg protein
Source:
Porcine Spleen
I.U.B.: 3.1.22.1
Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (
BBRC, 17
, 573, 1971) describes a three stage degradation of native DNA by DNase II.
Unit Definition
: One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.
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