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商品編號


R0168S-15,000 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


Restriction Endonucleases



Concentration

10,000 units/ml


Size

15,000 units

商品信息

Description:
Product Source
An
E. coli
strain that carries the cloned BstNI gene from
Bacillus stearo
Thermo
philus
N (D. Comb)
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? 3.1
-20
10X
Notes:
BstNI is an isoschizomer of EcoRII but cuts DNA at a different location. (EcoRII cuts before the two C residues).BstNI produces DNA fragments that have a single-base 5??extension which are more difficult to ligate than blunt-ended fragments. More efficient ligation can be achieved by using the Quick Ligatin Kit (
NEB
#M2200).The Activity of BstNI at 37°C is 30%.Based on the st
ABI
lity of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch? PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.