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商品編號


R0138S-2,000 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


Restriction Endonucleases



Concentration

20,000 units/ml


Size

2,000 units

商品信息

Description:
SalI has a High Fidelity version SalI-HF? (
NEB
#R3138).
High Fidelity (HF?) Restriction Enzymes have 100% activity in CutSmart??Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. ?HF enzymes also exhibit dramatically reduced star activity. ?HF enzymes are all Time-Saver? qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. ?Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. ?
Product Source
An
E. coli
strain that carries the SalI gene from
Streptomyces albus
G (
ATCC
49789).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? 3.1
-20
10X
Notes:
This enzyme has shown to have lower activity on some supercoiledplasmids, with more than 1 unit required to digest 1 μg plasmid DNA. Forcomplete digestion of 1 μg of plasmid DNA please follow our recommendeddigestion protocol.Cleavage of mammalian genomic DNA is blocked by CpGmethylation.