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商品編號


R0627S-2,500 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


Restriction Endonucleases



Concentration

10,000 units/ml


Size

2,500 units

商品信息

Description:
Nt.AlwI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate. It is an engineered derivative of AlwI which catalyzes a single strand break 4 bases beyond the 3? end of the recognition sequence on the top strand.
Product Source
An?
E. coli?
strain containing a chimeric gene encoding the DNA recognition domain of AlwI and the cleavage/dimerization domain of Nt.BstNBI.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation.To see how this enzyme (along with other nicking enzymes) performs at different temperatures, please visit Effect of Various Temperatures of Different Nicking Endonucleases.