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商品編號


LS01132



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分類(lèi)


Products



Size

5 gm

商品信息
DNA Cellulose, Single-StrandedSource: Calf Thym
USP
repared by a method developed at
Worthington
in which denatured, single-stranded calf thymus DNA is covalently bound to cellulose. Suitable for the purification of many proteins that are associated with nucleic acids such as DNA/RNA polymerases and endo- and exo-nucleases and reverse transcriptases. Supplied as a dry powder. One gram of DNA-cellulose will swell to 3-4ml when fully hydrated.Store at 2-8°C.≥3 mg DNA per gm dry weight
Worthington
offers DNA purified from these sources:
Calf thymus
: (Code: DNA) Prepared and purified by a method developed at
Worthington
to have lower protein and RNA contamination than most other commercial preparations. This highly polymerized DNA is an excellent substrate for deoxyribonuclease. A sodium salt, it must be converted by adding magnesium ions to be suscept
IBL
e to DNase.
Calf thymus DNA, covalently bound to cellulose
is also available:
DNA Cellulose, Double-Stranded
(Code: DNACELDS)
DNA Cellulose, Single-Stranded
(Code: DNACELSS)
Salmon Testes
: (Code: SDNA) Prepared by a modification of the method of Emanuel and Chaikoff,
J. Biol. Chem., 203
, 164, (1953). A minimum of 75% native nucleic acid.
Salmon Testes DNA, Denatured & Fragmented
(Code: SDNAD) is also available. Prepared by mechanical shearing and heat denaturation.
Escherichia coli
: (Code: DNAEC) Isolated as described by Marmur,
J. Mol. Biol., 3
, 208, 1961.
Lam
BD
a phage DNA
(Code: DNAL) is prepared from CsCl purified phage and is purified to an A
260
/A
280
≥1.8. Homogeneous in agarose gel electrophoresis. A solution in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.
DNA fragments
prepared by restriction endonuclease digestion of purified lam
BD
a DNA are available (Codes: DNALBSTE; DNALECOR; DNALHIND). Supplied as solutions in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.
Technical Note
: One A
260
unit = 50 ?g DNA.
St
ABI
lity/Storage:
DNAL: Storage buffer 10 mM Tris-HCl, pH 8.0 containing 1 mM EDTA. Store at -20°C. ?Once thawed keep at 2-8°C.
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Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
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E?RASE RNase A/T1 Blend (RCT)
Histones (H, NHL)
Lysozyme (LY/LYSF)?
Nuclease, Micrococcal (NFCP)
Nuclease, S1 (SINUC/SINUCL)
Phosphatase, Alkaline (CAP/BAPF/BAPC/BAPSF/PC)
Phosphodiesterase I (VPH)?
Phosphodiesterase II (SPH)?
Proteinase K (PROK/PROKS)
Reverse Transcriptase, Recombinant HIV (RTHIV)?
Ribonuclease A (R/RAF/RASE/RS/RPDF)
Ribonuclease T1, Animal Origin Free (RT1S)