NEB/SalI-HF?/R3138S/10,000 units
克隆和表達
商品編號
R3138S-10,000 units
品牌
紐英倫(NEB)
公司
New England Biolabs
公司分類(lèi)
Epigenetics
Concentration
20,000 units/ml
Size
10,000 units
商品信息
Description:
??
High Fidelity (HF?) Restriction Enzymes have 100% activity in CutSmart? Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.HF enzymes also exhibit dramatically reduced star activity.HF enzymes are all Time-Saver? qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA.Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
NEB
extensively performs quality controls on all standard and high-fidelity(HF?) restriction enzymes. Examples of nuclease contamination studies for someof our HF restriction enzymes are shown below.
Restriction Enzyme Competitor Study: Nuclease Contamination
EcoRI, NotI, and BamHI from multiple suppliers were tested in reactionscontaining a fluorescent labeled single stranded, double stranded blunt,3’overhang or 5’ overhang containing oligonucleotides. The percent degradationis determined by capillary electrophoresis and peak analysis. The resolution isat the single nucleotide level.
Product Source
An?
E. coli?
strain that carries the cloned and modified SalI gene from?
Streptomyces albus
?G (
ATCC
49789)
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Gel Lo
ADI
ng Dye, Purple (6X)
25
6X
產(chǎn)品貨號:2655.2