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商品編號


R0147S-500 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


Restriction Endonucleases



Concentration

5,000 units/ml


Size

500 units

商品信息

Description:
Product Source
An
E. coli
strain that carries the MboI gene from
Moraxella bovis
(
ATCC
10900).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
BfuCI, DpnII and Sau3AI are isoschizomers of MboI.MboI cleaves to leave a 5??GATC extension which can be efficiently ligated into BamHI, BclI, BglII, BstYI, DpnII or Sau3AI cleaved fragments.MboI is blocked by dam methylation, however Sau3AI is not sensitive to dam methylation.This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation.Based on the st
ABI
lity of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.