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商品編號


R0646S-500 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


Restriction Endonucleases



Concentration

10,000 units/ml


Size

500 units

商品信息

Description:
Product Source
An?
E. coli
?strain that carries the cloned EcoP15 l?res-mod?genes from plasmid pSHl180 (D.N. Rao)
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
10X ATP
10X
NEB
uffer? 3.1
-20
10X
Notes:
ATP is required for DNA cleavage. Efficient cleavage requires the presence of two inversely oriented recognition sites. A head to head orientation is preferred. The "head" of the sequence is defined as the dG at the 3??end of the CAGCAG strand (top strand). Cleavage efficiency is also affected by the distance between the two sites.EcoP15I requires two copies of its recognition sequence for cleavage tooccur, with limited cleavage on plasmids with a single site.Based on the st
ABI
lity of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.