Biohelix/Thermostable Helicase/H0240S/20 ?g
克隆和表達
商品編號
H0240S
品牌
Biohelix
公司
BIO-HELIX Co., LTD.
公司分類(lèi)
Helicases
Size
20 ?g
Concentration
0.5 mg/ml
商品信息
Description:
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Thermo
stable DNA Helicase is a DNA helicase that uses the energy
generated by the hydrolysis of nucleoside triphosphates to break
the hydrogen bonds linking the two strands together in duplex DNA.
It is active in a temperature range of 45 to 65?C.
SUPPLIED WITH:
10X Annealing Buffer II
100 mM MgSO
4
Supplied by Users
: ATP
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Source:
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Purified from an
E. coli
strain that overexpresses the helicase
gene isolated from a
Thermo
philic microorganism.
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Reaction condition:
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1X Annealing Buffer II:
10 mM KCl
20 mM Tris-HCl, pH 8.8 @ 25°C
Supplement with 4 mM MgSO
4
and 3 mM ATP.
Incubate at 45 - 65?C.
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Storage Conditions:
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Storage Temperature:
-20?C
Concentration:
0.5 mg/ml
Storage Buffer:
20 mM Tris-HCl pH7.4
200 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
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Quality Control:
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Quality Assurance Statement:
Thermo
stable Helicase is purified free of contaminating endonucleases
and exonucleases. Each lot is tested for DNA unwinding activity.
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產(chǎn)品貨號:6261.6