服務(wù)熱線(xiàn)：
Biohelix/T7 Gp4A Helicase/H0250S/50 ?g
克隆和表達
商品編號
H0250S
品牌
Biohelix
公司
BIO-HELIX Co., LTD.
公司分類(lèi)
Helicases
Size
50 ?g
Concentration
1 mg/ml
商品信息
Description:
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The T7 Gp4A Helicase exhibits a 5? to 3? helicase activity
as well as primase activity. It is highly processive, traveling
an average of 75 kb of single stranded DNA before dissociating.
SUPPLIED WITH:
10X Gp4A Reaction Buffer
Supplied by Users
: dTTP
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Source:
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Purified from an
E. coli
strain that overexpresses the
gp4A
gene isolated from the T7 phage.
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Reaction condition:
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1X Gp4A Reaction Buffer:
35 mM Tris?acetate, pH 7.5
11 mM magnesium acetate
5 mM DTT
0.01% Triton X-100
Supplement with 2 mM dTTP.
Incubate at 25 - 37?C.
NOTE:
The concentration
of dTTP should be optimized for each specific application.
For the
primase activity, supplement reactions with NTPs.
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Storage Conditions:
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Storage Temperature:
-20?C
Concentration:
1 mg/ml
Storage Buffer:
20 mM TrisHCl pH 7.5
100 mM NaCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
pH 7.5 @ RT
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Quality Control:
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Quality Assurance Statement:
T7 Gp4A Helicase is purified free of contaminating endonucleases
and exonucleases. Each lot is tested for DNA unwinding activity in functional activity assays.
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產(chǎn)品貨號：6261.6
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