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商品編號


M0493S-500 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


PCR, qPCR & Amplification Technologies




500 units


Concentration

2,000 units/ml



商品信息

Description:
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Q5? Hot Start High-Fidelity DNA Polymerase is a high-fidelity,
Thermo
stable, hot start DNA polymerase with 3?→ 5? exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of
Taq
DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg
++
at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the
NEB
T
m
Calculatoris highly recommended.
Amplification of a variety of human genomic amplicons fromlow to high GC content using Q5 Hot Start High-Fidelity DNA Polymerase. Allreactions were set up at room temperature, conducted using 30 cycles ofamplification and visualized by microfluidic LabChip? analysis.
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Product Source
An
E. coli
strain that carries the Q5 High-Fidelity DNA Polymerase gene.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Q5? High GC Enhancer
5X
Q5? Reaction Buffer Pack
-20
5X