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商品編號


M0537S-8,000 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


PCR, qPCR & Amplification Technologies




8,000 units


Concentration

8,000 units/ml



商品信息

Description:

Bst
2.0 DNA Polymerase is an
in silico
designed homologue of
Bacillus stearo
Thermo
philus
DNA Polymerase I, Large Fragment (
Bst
DNA Polymerase, Large Fragment).
Bst
2.0 DNA Polymerase contains 5?→3? DNA polymerase activity and strong strand displacement activity but lacks 5?→3? exonuclease activity.
Bst
2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and
Thermo
st
ABI
lity compared to wild-type
Bst
DNA Polymerase, Large Fragment.
LAMP Salt Tolerance
Performing LAMP reactions with increasing amounts of salt demonstrate increased resistance to inhibitors by using Bst 2.0. Bst 2.0 maintains high levels of activity where wild-type Bst DNA polymerase, large fragment, is completely inhibited.
Rapid LAMP Detection
LAMP reactions amplifying different targets demonstrate a more rapid amplification threshold time using Bst 2.0 vs. Bst, large fragment.
Product Source
Bst
2.0 DNA Polymerase is prepared from an
E. coli
strain that expresses the
Bst
2.0 DNA Polymerase protein from an induc
IBL
e promoter.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Isothermal Amplification Buffer Pack
-20
10X
Magnesium Sulfate (MgSO
4
) Solution
-20
100 mM
Notes:
Bst 2.0 DNA Polymerase does not exhibit 3?→ 5? exonuclease activity.Reaction temperatures above 70°C are not recommended.Bst 2.0 DNA Polymerase cannot be used for thermal cycle sequencing or PCR.Specific reaction conditions will vary for different isothermal amplification applications. For best results, use 1X Isothermal Amplification Buffer.