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商品編號


R0104S-50,000 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


Restriction Endonucleases



Concentration

20,000 units/ml


Size

50,000 units

商品信息

Description:
HindIII has a?High Fidelity version HindIII-HF? (
NEB
#R3104).
High Fidelity (HF ?) Restriction Enzymes have 100% activity in CutSmart ? Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.HF enzymes also exhibit dramatically reduced star activity.HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA.Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
HindIII crystals (Ira Schildkraut and Lydia Dorner,
New England Biolabs
)
Product Source
An
E. coli
strain that carries the HindIII gene from
Haemophilus influenzae
Rd (
ATCC
51907).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? 2.1
-20
10X
Notes:
Not sensitive to dam, dcm or mammalian CpG methylation.This enzyme has shown to have lower activity on some supercoiledplasmids, with more than 1 unit required to digest 1 μg plasmid DNA. Forcomplete digestion of 1 μg of plasmid DNA please follow our recommendeddigestion protocol.Star activity may result from extended digestion.