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商品編號


M0257S-1,000 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)






1,000 units


Concentration

2,000 units/ml

商品信息

Description:
Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3?→ 5? proofre
ADI
ng exonuclease activity associated with Vent DNA Polymerase (1). This?is the preferred form for high-temperature dideoxy sequencing reactions and for high yield primer extension reactions. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of
Taq
DNA Polymerase (2,3).
Highlights
Isolated from a recombinant source
Supplied with 10X Reaction Buffer and MgSO
4
High
Thermo
st
ABI
lity: half-life of 6.7 hours at 95°C
Product Source
An?
E. coli
?strain that carries the Vent (D141A / E143A) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase from?
Thermo
coccus litoralis
?(4). The native organism is capable of growth at up to 98°C and was isolated from a su
BMA
rine thermal vent (5).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Magnesium Sulfate (MgSO
4
) Solution
-20
100 mM
Thermo
Pol
??
Reaction Buffer Pack
-20
10X
Notes:
BSA is not provided with this enzyme since its presence is not necessary for most primer extension reactions. However, it is available as
NEB
#B9001. Acetylated BSA should not be used for primer extension reactions.Diluent D is also available. This diluent is recommended for making dilutions of Vent (exo-) Polymerase.Additional
Thermo
Pol Reaction Buffer Packs for this product are also available. Each buffer pack contains 4 vials of 10X buffer (1.5 ml each) and 1 vial of 100 mM MgSO4.