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商品編號


M0495S-1,000 units



品牌


紐英倫(NEB)



公司


New England Biolabs



公司分類(lèi)


PCR, qPCR & Amplification Technologies




1,000 units


Concentration

5,000 units/ml

商品信息

Description:
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Hot Start
Taq
DNA Polymerase is a mixture of
Taq
DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds revers
IBL
y to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme.
Taq
DNA polymerase possesses a 5?→3? polymerase activity
(1)
(2)
(3)
and a 5? flap endonuclease activity
(4)
(5)
.
It is supplied with 10X Standard
Taq
Reaction Buffer, which is detergent-free and designed to be compat
IBL
e with existing assay systems.
Product Source
An
E. coli
strain that carries the
Taq
DNA Polymerase gene from
Thermus aquaticus
YT-1.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Standard
Taq
Reaction Buffer Pack
-20
10X