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商品編號


30085-3



品牌


Lucigen



公司


Lucigen



公司分類(lèi)


Polymerases




1500 U

商品信息


Only source for recombinant T4 DNA Polymerase, Exonuclease Minus.

Potential applications include enhancing DNA sequencing reads.




T4 DNA Polymerase, Exonuclease Minus is a DNA-dependent DNA polymerase which catalyzes the 5’→3’ synthesis of DNA and requires template and primer.? Additionally, the enzyme lacks 3’→5’ exonuclease activity.


Source:
Purified from a strain of
E. coli
that carries a T4 DNA Polymerase, Exonuclease Minus expression plasmid.


Applications:


DNA Sequencing reaction.(1)



Heat inactivation:
70°C for 15 minutes.


Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.


Additional Information:


May increase the DNA sequencing read-lengths.



References


Nancy G. Nossal (1998)
Genetics
148:
1535-1538.


ORDER INFORMATION
Recombinant T4 DNA Polymerase, Exonuclease Minus is supplied at a concentration of 3,000U/ml in a storage buffer of 50% glycerol, 100 mM KPO4 (pH 6.5) and 1 mM DTT. Also supplied is 10X DNA Polymerase Buffer A composed of 100 mM Tris-HCl (pH7.9), 100 mM MgCl2, 500 mM NaCl and 10 mM DTT.