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商品編號


30222-1



品牌


Lucigen



公司


Lucigen



公司分類(lèi)


Reverse Transcriptase Enzymes



Size

50,000 U


商品信息



Outstanding
CDN
A synthesis.





M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofre
ADI
ng function. This enzyme can copy a single-stranded DNA template or perform
CDN
A synthesis by extending a DNA primer annealed to an RNA template.

Specifications

Unit Definition:
1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
Source:
A recombinant
E. coli
strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.







Unit Concentration

200,000 U/mL



Purity (SDS-PAGE)

>99%



SS Exonuclease

2,000 U <5.0% released



DS Exonuclease

2,000 U <0.5% released



Endonuclease

2,000 U <10% converted



E. coli
16S rDNA Contamination

2,000 U <10 copies



Storage

-20°C



ORDER INFORMATION
Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25°C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl
2
, 10 mM dithiothreitol, pH 8.3 @ 25°C. 250,000 U is supplied as 5 × 50,000 U.