欧美自拍另类欧美宗合图片区,国产视频一区二区三区四区,日本一区二区三区四区视频,婬片一区AAA毛片一区二区

商品編號


P7390L



品牌


Enzymatics



公司


Enzymatics,inc



公司分類(lèi)


New Products



Size

40,000 U

商品信息

Bst X DNA Polymerase


Product Description

Bst X DNA Polymerase is a
Thermo
stable DNA polymerase from Geobacillus, homologous to the DNA polymerase from
Bacillus stearo
Thermo
philus
(Bst). Bst X lacks 5’→3′ and 3’→5′ exonuclease activity and exhibits a stronger strand-displacement activity than Manta 1.0 DNA Polymerase that may allow it to perform better in isothermal amplification technologies.

Usage Notes:


Optimal reaction temperature from 60-70°C.

Heat inactivated at 80°C after 10 min incubation.

High Tolerance to the non-ionic detergents Tween-20, Tween-80, TX-100, and NP-40 (up to 5% final concentration).

Active in salt from 40 to 125 mM.


Buffer Description
10x Bst X Xcelerator Reaction Buffer, contains 2 mM MgSO
4
at 1X concentration. Depending on the application, it may be necessary to adjust the MgSO
4
from 2-10 mM final concentration.

Source of Protein
A recombinant
E.coli
strain carrying the Bst X DNA Polymerase (exo-) polymerase gene.

Unit Definition
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C

Molecular weight
66,494 Daltons



Quality Control Analysis

Unit

Activity
is measured using a 2-fold serial dilution method. Dilutions of enzyme batch were made in 1X reaction buffer and added to 50 μL reactions containing Calf Thymus DNA, 1X PCR Buffer II, 3H-dTTP and 100 μM dNTPs. Reactions were incubated 10 minutes at 65°C, plunged on ice, and analyzed using the method of Sambrook and Russell (Molecular Cloning, v3, 2001, pp. A8.25-A8.26).

Protein

Concentration
is determined by OD
280
absorbance.

Physical Purity
is evaluated by SDS-PAGE of concentrated and diluted enzyme solutions followed by silver stain detection. Purity is assessed by comparing the aggregate mass of contaminant bands in the concentrated sample to the mass of the protein of interest band in the diluted sample.

Single-Stranded

Exonuclease
is determined in a 50 ?L reaction containing a r
ADI
olabeled single-stranded DNA substrate and 10?L of enzyme solution incubated for 4 hours at 37°C.

Double-Stranded Exonuclease
is determined in a 50 ?l reaction containing a r
ADI
olabeled double-stranded DNA substrate and 10 ?L of enzyme solution incubated for 4 hours at 37°C.

Double-Stranded Endonuclease
is determined in a 50 ?L reaction containing 0.5 ?g of plasmid DNA and 10 ?L of enzyme solution incubated for 4 hours at 37°C.

E.coli
16S rDNA Contamination
is evaluated using 5 ?L
r
eplicate samples of enzyme solution denatured and screened in a TaqMan qPCR assay for the presence of contaminating
E.coli
genomic DNA using oligonucleotide primers corresponding to the 16S rRNA locus.

Supplied in
50 mM KCl
1mM DTT
0.1mM EDTA
0.1% Triton X-100
50% glycerol
10mM Tris-HCl
pH 7.5 @ 25°C.


View PDF Poster Instructions FAQ


Product Information



Bst X DNA Polymerase


Part Number
P7390L
Price
$513
Concentration
40,000 U/mL
Unit Size
40,000 U


SDS

Available on request








Product Specification*


Storage Temperature
-25 to -15°C


Test

Units Tested

Specification


SDS Purity
N/A
>99%
Specific Activity
N/A
400,000 U/mg
SS Exonuclease
4000 U
<5.0% released
DS Exonuclease
4000 U
<1.0% released
DS Endonuclease
4000 U
No conversion
E.coli
DNA Contamination
4000 U
< 10 copies





* For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section.

Additional Downloads

Application Note

Notes

Alternate Temperature


Approximately 15% activity at 37°C.

Approximately 5% activity at 25°C.


Legal Disclaimer

Patents
Certain applications in which this product can be used may be covered by patents issued and applicable in the United States and abroad. Purchase of this product does not include a license to perform any patented application; therefore it is the sole responsibility of users of this product to determine whether they may be required to engage a license agreement depending upon the particular application in which the product is used.



Limitations of Use
This product was developed, manufactured, and sold for in vitro use only. The product is not suitable for admi
NIST
ration to humans or animals. SDS sheets relevant to this product are available upon request.