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商品編號


T3DL-OEM



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


DNA/RNA Ligases



Size

Any Size

商品信息

T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl terminus in duplex DNA. The enzyme will join blunt ends and cohesive ends as well as repair single stranded nicks in duplex DNA.




Description:
T3 DNA Ligase joins blunt ends and cohesive ends as well as repairs single-stranded nicks in duplex DNA. In the absence of 20-30% PEG 6000, T3 DNA Ligase displays a very low efficiency for blunt-ended ligation
(1)
. T3 DNA Ligase displays a higher efficiency for joining A/T overhangs than C/G matched ends
(1)
. T3 DNA Ligase retains 95% of its activity in 1.0 M NaCl or KCl, with an optimal concentration of 300 mM
(1)
.
Application:
Catalyzes the formation of a phosphodiester bond between a 5'-phosphate and a 3'-hydroxyl terminus in duplex DNA.
Source:
A recombinant
E. coli
strain carrying the T3 DNA Ligase gene.
Supplied in:
20 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C
Supplied with:
2x Rapid Ligation Buffer
2x Rapid Ligation Buffer:
132 mM Tris-HCl
20 mM MgCl
2
2 mM DTT
2 mM ATP
15% PEG 8000
pH 7.6
?


Unit Definition:
One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [
32
PP
i
] into Norit-adsorbable form in 20 min at 37°C.? Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl
2
, 0.066 mM ATP, 10 mM DTT, 3.3 ?M [
32
PP
i
].


One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit.


Specific Activity:
?30,000 Weiss Unit/
m
g


Recommended Storage Condition:
-20°C
Reference:
1. Cai, Liang, et al. (2004) J. Biochem. 135, 397-403