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商品編號


TL-050



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


DNA/RNA Ligases



Concentration

5 U/μl


Size

250 Weiss units

商品信息

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA using ATP as a cofactor.




Description:
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA using ATP as a cofactor.


Figure.
Ligation of λ DNA/Hind III fragments analyzed by agarose gel electrophoresis.
Performances of T4 DNA ligase from
MCLAB
and another supplier were compared within identical reaction mixture at 37°C for 30 minutes. Paralleled negative control was run without ligase.


Application:
- Cloning of restriction fragments
- Joining linkers and adapters to blunt-ended DNA
Source:
A recombinant
E. coli
strain carrying the cloned T4 DNA Ligase gene.
?


Supplied In:
10 mM Tris-HCl
50 mM KCl?
1 mM DTT
0.1 mM EDTA?
50% Glycerol?
pH 7.4 @ 25°C
Supplied With:
10x T4 DNA Ligase Buffer
500mM Tris-HCl
100 mM MgCl
2
50 mM DTT
10 mM ATP
pH 7.6 @ 25°C
Unit Definition:
One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [
32
PP
i
] into Norit-adsorbable form in 20 min at 37°C.? Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl
2
, 0.066 mM ATP, 10 mM DTT, 3.3 ?M [
32
PP
i
].


One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit.


Specific Activity:
300U/
?
g


Recommended Storage Condition:?
-20°C


Reference:
1. Engler, M.J. and Richardson, C.C. (1982) P.D. Boyer (Eds.), The Enzymes, 5, pp. 3. San Diego: Academic Press.