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商品編號


T4RL2-100



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


DNA/RNA Ligases



Concentration

10,000 U/ml


Size

2,500 units

商品信息

T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA. The enzyme requires an adjacent 5? phosphate and 3? OH for ligation.




Description:
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), catalyzes both inter- and intramolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 is much more active on joining nicks on double stranded RNA than on joining the ends of single-stranded RNA. The enzyme requires an adjacent 5'-phosphate and 3'-OH for ligation. The enzyme can also ligate the 3'-OH of RNA to the 5'-phosphate of DNA in a double stranded structure.


Source:
An
E. coli
strain that carries the T4 RNA Ligase 2 gene (I. Schildkraut).
Application:
- Ligates a nick in dsRNA
- Ligates the 3'-OH of RNA to the 5'-phosphate of DNA in a double stranded structure
Reagents Supplied:
T4 Rnl2 Reaction Buffer
Reaction Conditions:
1x T4 Rnl2 Reaction Buffer
Incubate at 37°C
10x T4 RNA Ligase 2 Reaction Buffer:
500 mM Tris-HCl
20 mM MgCl
2
10 mM DTT
4 mM ATP
pH 7.5 @ 25°C
Unit Definition:
One unit is defined as the amount of enzyme required to ligate 0.4 ?g of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 ?l in 30 minutes at 37°C.
Unit Assay Conditions:
1x T4 Rnl2 Reaction Buffer and 0.4 ?g of an equimolar mix of the 23-mer and 17-mer RNAs. After incubation at 37°C for 30 minutes, the ligated product is detected on a 15% polyacrylamide gel.


Specific Activity:
>40,000U/mg
?


Storage Conditions:
10 mM Tris-HCl
50 mM KCl
35 mM (NH
4
)
2
SO
4
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C
Recommended Storage Condition:
-20°C