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商品編號


T7DL-300



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


DNA/RNA Ligases



Concentration

45,000 Weiss U/ml


Size

45,000 Weiss units

商品信息

T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA.




Description:
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5'-phosphate and a 3'-hydroxyl termini in duplex DNA. The enzyme will join blunt ends and cohesive ends termini as well as repair single-stranded nicks in duplex DNA.
Application:
- Joining of Okazaki fragments during replication
- Completing short-patch DNA synthesis occurring in DNA repair process
Source:
A recombinant
E. coli
strain carrying the T7 DNA Ligase gene.
Supplied in:
20 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C
10x Rapid Ligation Buffer:
660 mM Tris-HCl
100 mM MgCl
2
10 mM DTT
10 mM ATP
75% PEG 6000
pH 7.6 @ 25°C


Unit Definition:
One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [
32
PP
i
] into Norit-adsorbable form in 20 min at 37°C.? Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl
2
, 0.066 mM ATP, 10 mM DTT, 3.3 ?M [
32
PP
i
].


One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit.


Specific Activity:
?45 Weiss U/
?
g


Recommended Storage Condition:
-20°C
Reference:
Doherty, A. et al. J.Biol. Chem (1996) V.271, No.19, 11083-11089.