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商品編號


RNHE-200



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


RNases



Concentration

10,000 U/ml


Size

10,000 units

商品信息

RNase H is an endoribonuclease that degrades the RNA strand of RNA/DNA hybrid molecules.




Description:
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5'-phosphate and 3'-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
Specific Activity:
625,000 U/mg
Application:
- Removal of poly(A) tails of mRNA hybridized to poly(dT)
- Removal of mRNA during second strand
CDN
A synthesis
Source:
A recombinant
E. coli
strain carrying the RNAse H gene (rnh) from
E. coli
.


Supplied in:
20 mM Tris-HCl
100 mM KCl
10 mM MgCl
2
0.1 mM EDTA
0.1 mM DTT
50% Glycerol
pH 7.9 @ 25°C
Supplied with:
10x RNAse H Buffer
10x RNAse H Buffer:
500 mM Tris-HCl
750 mM KCl
30 mM MgCl
2
100 mM DTT
pH 8.3 @ 25°C
Unit Definition:
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an
3
H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.
Recommended Storage Condition:
-20°C