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商品編號


HIND-300



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Restriction Endonucleases



Size

200,000 units, 200 units/μl

商品信息

A^AGCTT





Recognition site:
A^AGCTT
Source:
Purified from a strain of
E. coli
that bears the cloned Hind III gene.
Storage Buffer:
10 mM Tris-HCl (pH 7.4)
200 mM NaCl
0.5 mM EDTA
1mM DTT
0.5 mg/ml BSA
50% (v/v) Glycerol
10x Hind III Buffer
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl
2
100 μg/ml BSA
pH 7.9 @ 25°C
Reaction Conditions:
Buffer at 37°C.
Typical Ligation/Recut Assay Results:
Hind III-cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by Hind III.
Recommended Storage Condition:
-20°C.
Comments:
Does not cleave DNA when either the 5'A or the C residue is N6 –methyladenine or 5-methylcytosine, respectively. Resistant to heat inactivation (10 minutes, 65°C).