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商品編號


PST-300



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Restriction Endonucleases



Size

200,000 units, 200 units/μl

商品信息


PstI, BspMAI





Recognition site:
CTGCA^G
Source:
Purified from a strain of
E. coli
that bears the cloned Pst I gene.
Storage Buffer:
10 mM Tris-HCl (pH 7.4), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) Triton X-100.
10x Pst I Buffer
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl
2
100 μg/ml BSA
pH 7.9 @ 25°C
Reaction Condition:
Buffer at 37°C.
Typical Ligation/Recut Assay Results:
Pst I-cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by Pst I.
Recommended Storage Condition:
-20°C.
Comments:
Does not cleave DNA when either the A or the 5’ C residue is N6 –methyladenine or 5-methylcytosine, respectively. Partially resistant to heat inactivation (10 minutes, 65°C). Glycerol concentrations >5% (v/v) may alter specificity.