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商品編號


HT-210



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Thermophilic DNA Polymerases



Size

5,000 Units,5 U/μl

商品信息

HoTaq? DNA Polymerase is a hot-start
Taq
DNA Polymerase, which is chemically modified form
Taq
DNA Polymerase.




Description:
HoTaq? DNA Polymerase is a hot-start
Taq
DNA Polymerase, which is a chemically?modified form of
Taq
DNA Polymerase. HoTaq DNA Polymerase is provided in an inactive state and has a minimum enzymatic activity at ambient temperatures. It will become active after 10 minutes heating at 95?C. This prevents the formation of misprimed products during reaction setup and the first denaturation step, leads to high PCR specificity. It is suitable for diagnostic reaction without the miner band. The enzyme is a high processive 5'-> 3' DNA polymerase that lacks 3'-> 5' exonuclease activity. Each lot of HoTaq DNA polymerase is tested for PCR amplification.
Supplied with:
10x
Taq
PCR Buffer (No dNTP)
Supplied in:
20 mM Tris-HCl (pH 8.0)
100 mM KCl
0.5% Tween 20
0.1 mM EDTA
1 mM DTT
50% (v/v) glycerol
Comparison:
Here is the result of comparing
MCLAB
's HoTaq with other le
ADI
ng brands.
Source:
An
E. coli
strain that carries the
Taq
DNA Polymerase gene from
Thermus aquaticus
(same as
Taq
DNA Polymerase).
Recommended Storage Condition :
-20°C
Recommended Reaction Conditions:
95°C, 10 minutes. -> (95°C, 10 seconds. -> 55°C, 30 seconds. ->72°C, 30 seconds.) for 25 cycles.
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C.
References:
1. Chien A, Edgar DB, Trela JM (1976). "Deoxyribonucleic acid polymerase from the extreme
Thermo
phile Thermus aquaticus". J. Bact. 127 (3): 1550–7. PMC 232952. PMID 8432.
2. Saiki, RK; et al. (1988). "Primer-directed enzymatic amplification of DNA with a
Thermo
stable DNA polymerase.". Science 239 (4839): 487–91. doi:10.1126/science.2448875. PMID 2448875.
3. Saiki, RK; et al. (1985). "Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia". Science 230 (4732): 1350–4. doi:10.1126/science.2999980. PMID 2999980.
3. Lawyer FC, et al. (1993). "High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase ...". PCR Methods Appl. 2 (4): 275–87. PMID 8324500.