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商品編號


PDP-200



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


PCR Polymerases



Description

1,250 Units,2.5 U/μl


Size

1 Ea

商品信息

I-5 Hi-Fi DNA polymerase is an ultra-fast and high-fidelity DNA polymerase. It provides robust amplification from different templates including plasmids, BACS, genomic DNA, and lam
BD
a DNA.




General Description
I-5 Hi-Fi DNA polymerase is an ultra-fast and high-fidelity DNA polymerase. It provides robust amplification of from different templates including plasmids, BACS, genomic DNA, and lam
BD
a DNA. It allows for amplification of up to 8kb with human genomic DNA and up to 21kb with lam
BD
a DNA. It has an extension speed of 1 kb / 10-15 seconds depending on template type. This allows the user to save time by speeding up PCR reactions and provides higher fidelity than
Taq
or
Pfu
.
Feature
? Robust – maximal success and minimal optimization needed
? Fidelity – 50X greater than
Taq
? High Speed –10X faster than
Pfu
? High Yield
? Versatile – best for long or difficult templates
Source
E. coli


Applications
PCR
Cloning
Long or Difficult Amplification
High-Throughput PCR


Supplied with
5X Reaction Buffer (1ml), 50mM MgCl
2
(1ml)


Supplied in (buffer description)
20mM Tris-HCl, 100mM KCl, 1mM DTT, 0.1mM EDTA, 200ug/ml BSA, 50% glycerol, 1X st
ABI
lizer, pH 7.4 @ 25 C


Storage Condition
-20°C


Unit Definition
One unit is the amount of enzyme that incorporates 5 nmol of dNTPs into acid insoluble material in 15 minutes at 72°C.


References?
Frey, B. and Suppman, B. (1995). BioChemica. 2, 34-35.
Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.
Shipping Condition
dry ice