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商品編號


DPTN-200



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Thermophilic DNA Polymerases



Concentration

5 U/μl


Size

5,000 units

商品信息

DNA polymerase from
Thermo
toga neapolitana
was identified[1] as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR[2].




Description:
Due to its
Thermo
stable nature,?
Thermo
toga neapolitana?
DNA polymerase (
Tne
) was identified
(1)
as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR
(2)
. Similar to
E. coli
DNA polymerase I, but unlike
Taq
DNA polymerase,
Tne
DNA polymerase contains both 3'-> 5' and 5'-> 3'- exonulease activities. Therefore, ?it replaced the DNA polymerase from
E. coli
originally used in PCR
(3)
.
Taq
's optimum temperature for activity is 75-80°C, with a half-life of 9 minutes at 97.5°C, and can replicate a 1000 bp strand of DNA in less than 10 seconds at 72°C
(4)
.

The DNA products have an A (adenine) overhangs at their 3' ends. This may be useful in TA cloning, whereby a cloning vector (such as a plasmid) that has a T (thymine) 3'- overhang is used, which complements with the A overhang of the PCR product, enabling ligation of the PCR product into the plasmid vector.
Application:
- PCR (ordinary and high-throughput)
- Primer Extension
- Microarray Analysis
- Denaturing high performance liquid chromatography (DHPLC)
Source:
Thermo
toga neapolitana
(
Tne
) DNA polymerase belongs to the DNA polymerase I (Pol I) family.
Recommended Reaction Conditions:
94°C, 1 minute. -> (94°C, 10 seconds. -> 55°C, 30 seconds. ->72°C, 30 seconds.) for 25 cycles.
Recommended Storage Condition:
-20?C
References:
1. Chien A, Edgar DB, Trela JM (1976). "Deoxyribonucleic acid polymerase from the extreme
Thermo
phile Thermus aquaticus". J. Bact. 127 (3): 1550–7. PMC 232952. PMID 8432.
2.Saiki, RK; et al. (1988). "Primer-directed enzymatic amplification of DNA with a
Thermo
stable DNA polymerase.". Science 239 (4839): 487–91. doi:10.1126/science.2448875. PMID 2448875.
3. Saiki, RK; et al. (1985). "Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia". Science 230 (4732): 1350– doi:10.1126/science.2999980. PMID 2999980.
4. Lawyer FC, et al. (1993). "High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase ...". PCR Methods Appl. 2 (4): 275–87. PMID 8324500.