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商品編號


AD-200



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Thermophilic DNA Polymerases



Concentration

2.5 U/μl


Size

500 Units

商品信息


Pfu
DNA Polymerase is a highly
Thermo
stable DNA polymerase from the hyper
Thermo
philic archaeum
Pyrococcus furiosus
.




Description:
Pfu
DNA Polymerase is a highly
Thermo
stable DNA polymerase from the hyper
Thermo
philic archaeum
Pyrococcus furiosus
. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'->3' direction.
Pfu
DNA Polymerase also exhibits 3'->5' exonuclease (proofre
ADI
ng) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5'->3' exonuclease activity. The main difference between
Pfu
and alternative enzymes is
Pfu
's superior
Thermo
st
ABI
lity and 'proofre
ADI
ng' properties. Unlike
Taq
DNA polymerase,
Pfu
DNA polymerase also possesses 3'->5' exonuclease proofre
ADI
ng activity, resulting in PCR fragments with fewer errors than Taq-generated PCR inserts.
Pfu
DNA polymerase is efficient for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with
Taq
polymerase to obtain the fidelity of
Pfu
with the speed of
Taq
polymerase activity.
Application:
- High-fidelity PCR and primer-extension reactions
- Generation of PCR products for cloning and expression
- PCR cloning and blunt-end amplification product generation
- RT-PCR for
CDN
A cloning and expression
- Site-directed mutagenesis
- Blunt-end PCR cloning
Source:

Thermo
stable DNA polymerase from hyper
Thermo
philic archaeon
pyrococcus furiosus
.
Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into acid insoluble material in 30 minutes at 74°C under standard DNA polymerase assay conditions.
Supplied With:
10x
Pfu
Reaction Buffer (with dNTPs)
?


10x Pfu Reaction Buffer (with dNTPs)?
200mM Tn3 pH 8.8
20 mM MgSO
4
100 mM KCl
100 mM (NH
4
)
2
SO
4
1% Triton
1 mg/ml BSA
2 mM dNTP


Supplied In:
20 mM Tris-HCl (pH 8.0)
40 mM NaCl
0.1 mM EDTA
1 mM DTT
50% (v/v) glycerol
Heat Inactivation:
95% inactive after 1-hour incubation at 98°C
Recommended Reaction Conditions:
1X
Pfu
buffer, 200 ?M each dNTP, 0.1-0.5?M each primer, 5 units
Pfu
DNA polymerase enzyme, 1-100ng plasmid template DNA, or 100-250ng genomic template DNA.
Recommended Storage Condition:
-20?C