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商品編號


20018708



品牌


宜曼達(Illumina)



公司


Illumina, Inc.



公司分類(lèi)


Library Preparation Kits


商品信息

Product Highlights:
The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.


Fastest
Illumina
library prep workflow, with ~3.5 hours total time

Flexibility to accommodate variations in sample type, DNA input amount, and application

Optimized library prep performance, generating reliable results


Save Time and Resources

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.


Simplify Lab Operations

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproduc
IBL
e sequencing data.

*Demonstrated protocols available.
Specifications:
Assay Time
~3.5 hours total assay time
Hands-On Time
1-1.5 hours
Input Quantity
Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng
Content Specifications
Human Whole Genome, Small Whole Genome, Large Whole Genome
Mechanism of Action
Bead-linked transposome
Multiplexing
Up to 96 available indexes
Species Category
Any Species
System Compatibility
MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class
Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Method
Whole-Genome Sequencing
Technology
Sequencing
Automation Cap
ABI
lity
Liquid Handling Robots