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商品編號


DPI-200



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Mesophilic DNA Polymerases



Concentration

10,000 U/ml


Size

10,000 units

商品信息

DNA Polymerase I is a?DNA-dependent DNA polymerase. The enzyme catalyzes 5' -> 3' synthesis of DNA and exhibits 3' -> 5’ exonuclease proofre
ADI
ng activity.




Description:
DNA Polymerase I is a mesophilic DNA polymerase that exhibits 5' -> 3' DNA synthesis activity in addition to both 3' -> 5' and 5' -> 3' exonuclease activities. The combination of DNA synthesis and 5' -> 3' nuclease characteristics enable nick-translation during DNA synthesis.
Application:
- Nick translation of DNA to obtain probes with a high specific activity
- Second strand synthesis of
CDN
A
Source:
A recombinant
E. coli
strain carrying the PolA gene.
Specific Activity:
6,850U/mg
Supplied in:
25 mM Tris-HCl
0.1 mM EDTA
1.0 mM DTTl
50% Glycerol
pH 7.4 @ 25°C
Supplied with:
10x Blue Buffer
10x Blue Buffer:
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl
2
10 mM DTT
pH 7.9 @ 25°C
Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.
Recommended Storage Condition:
-20°C