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商品編號


KPIM-100



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Mesophilic DNA Polymerases



Concentration

50,000 U/ml


Size

10,000 units

商品信息

Klenow Fragment, 3'->5' exo–, is the large fragment of
E.coli
DNA polymerase I with a molecular weight of 68kDa.




Description:
Klenow Fragment, 3'->5' exo–, is the Large Fragment of E.coli DNA Polymerase I with a molecular weight of 68kDa. It exhibits 5'->3' polymerase activity, but lacks the 3'->5' exonuclease activities. The 3'->5' exonuclease activity of the enzyme is eliminated by mutations in the 3'->5' exonuclease active site.
Specific Activity:
5,000U/mg
Application:
- Random priming labeling
- DNA sequencing by the Sanger dideoxy method
- Second strand
CDN
A synthesis
- Second strand synthesis in mutagenesis protocols
Supplied in:
20 mM Tris-HCl
100 mM NaCl
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C
Supplied with:
10x Blue Buffer
10x Blue Buffer:
500mM NaCl
100mM Tris-HCl
100mM MgCl
2
10mM DTT
pH 7.9 @ 25 ?C
Unit Definition:
1 unit is defined as the amount of enzyme that will incorporate 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30min at 37°C.
Recommended Storage Condition:
-20°C