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商品編號


PP-100



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Mesophilic DNA Polymerases



Concentration

10,000 U/ml


Size

1,000 units

商品信息

Phi29 DNA Polymerase is a replicative polymerase. This enzyme has??assist DNA replication, phi29 DNA Polymerase has the highest processivity and strand displacement activity among known DNA polymerases - more than 70 kb long DNA stretches can be synthesized.




Description:
Phi29 DNA Polymerase is respons
IBL
e for the replication of the
Bacillus Subtilis
phage phi29
(1)
. The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity
(2)
and a 3' -> 5' proofre
ADI
ng exonuclease function
(3)
.
Specific Activity:
100,000U/mg
Application:
- Catalyzes the removal of 5'-mononucleotides from duplex DNA
- Replication requiring a high degree of strand displacement and/or processive synthesis
- High fidelity replication at moderate temperatures
Source:
A recombinant
E. coli
strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.
Supplied in:
10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.5% Tween-20
0.5% NP-40
50% Glycerol
pH 7.4 @ 25°C
Supplied with:
10x phi29 DNA Polymerase Reaction Buffer
10x phi29 DNA Polymerase Buffer
500 mM Tris-HCl
100 mM (NH
4
)
2
SO
4
40 mM DTT
100 mM MgCl
2
pH 7.5 @ 25°C
Unit Definition:
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C.
Recommended Storage Condition:
-20°C
References:
1. Blanco, L. and Salas, M. (1984) Proc. Natl. Acad. Sci. USA, 81, 5325-5329.
2. Blanco, L. et al. (1989) J. Biol. Chem., 264, 8935-8940.
3. Garmendia, C. et al. (1992) J. Biol. Chem., 267, 2594-2599.