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商品編號


WG-321-1001



品牌


宜曼達(Illumina)



公司


Illumina, Inc.



公司分類(lèi)


Library Preparation Kits


商品信息

Product Highlights:
TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. The assay enables you to focus interrogation on key regions of genomic interest. Up to 1536 amplicons can be sequenced in a single reaction using a simple workflow. This highly targeted approach offers unparalleled efficiency for discovering, validating, and screening genetic variants in areas of research focus.

Requiring as little as 50 ng of input genomic DNA (gDNA), this kit can be used with a wide range of sample types. The
ABI
lity to multiplex up to 1536 amplicons per reaction allows coverage of up to 650 kb of cumulative sequence. Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling you to fully benefit from desktop sequencer output cap
ABI
lities.

This assay provides improved performance in difficult to address GC-rich regions, as well as increased library yield, uniformity, and st
ABI
lity. Additionally, optimized amplification steps enable sample input requirements to drop from 150 ng to 50 ng.

Simplified Probe Design

Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio
Start a project in DesignStudio now (login required)


Simple Data Analysis

Automated data analysis using the BaseSpace TruSeq Amplicon App allows researchers to easily review project data, including variants detected, across multiple runs.

Species Compatibility

TruSeq Custom Amplicon supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition,
Illumina
Concierge services can design custom assays and/or provide assay optimization support for any species of interest.
Inquire about
Illumina
Concierge services
Find an up-to-date list of high-throughput automation vendors with robotic systems compat
IBL
e with this library preparation kit
Specifications:
Assay Time
1 day
Hands-On Time
4 hours
Input Quantity
50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications
Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing
Up to 384 samples per sequencing run
Mechanism of Action
Amplification
Variant Class
Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)
System Compatibility
HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000
Species Category
Other,Bovine,Mouse,Human,Rat
Specialized Sample Types
FFPE
Technology
Sequencing
Automation Cap
ABI
lity
Liquid Handling Robots
Method
Amplicon Sequencing,Custom Sequencing