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商品編號


LVP473



品牌


Gentarget



公司


Gentarget



公司分類(lèi)


Pathway Reporter




1 Ea

商品信息

The pre-made lentivirus consitutively expresses a nuclear permeable
light-activator protein
under strong CMV promoter. It?is a photosensitive protein derived from Erythrobacter litoralis protein EL222. It consists of two domains, the light sensor domain (
LSD
or called
LOV
: light-oxygen-voltage) and the DNA binding domain (
D
BD

or called
HTH
: helix-turn-helix). In the dark, the two domain are interacted with each other to prevent its binding to “l(fā)ight induc
IBL
e promoter sequence”. When blue light is on (465nm), the
Illumina
tion breaks these interactions (protein formation changes), opening the D
BD
domain from LSD domain, which triggers the D
BD
binding to the light-induc
IBL
e promoter sequence, and turn on the downstream report expression.

This product?contains a
puromycin
?
selection
Marker
under RSV promoter. Click to?See?
Product Manual
?for details (.pdf).

Amount:
200 ul/per vial in DEME medium /10% FBS/ 10x Polybrene

CAT#:
LVP473