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商品編號


LVP304-PBS



品牌


Gentarget



公司


Gentarget



公司分類(lèi)


CRE recombinase




1 Ea

商品信息


Pre-made lentiviral particles
bicistronically
express two
individual
proteins:
firefly luciferase
and
nuclear permeable
CRE?recombinase
,
under the same suCMV promoter. A
Blasticidin
Marker
was expressed under RSV promoter, which allow to select the positive transduced stable cells for long term culture via antibiotic selection.

Both
lucifease
and
CRE
are highly active, best for both enzymes' assays
In vivo
or
In vitro
.
CRE
protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences,?named
lox sites
. CRE lentiviral particles?can?catalyze?the jointing event
In Vivo
?in the presence of?lox sites. This?
nuclear permeant?CRE (NLS-CRE)
?increases the recombination events
in vivo.
?Purified CRE enzyme can joint individual plasmids containing lox sites.


Luciferae
and NLS-
CRE
?are constitutively expressed in high level, and optionally, also can be used as tetracycline induc
IBL
e expression?as desired. Luciferase signal provides a convenient, realtime monitoring the?particles performance.???

This particles is concentrated and buffer changed into
PBS solution
, best suitable for hard-to-transduced cell lines, or serum-sensitive cell lines and in vivo application.

See
product manual
for details (.pdf).

Amount:??
200ul/per vial, at?5 x 10
7
IFU/ml in PBS
.?

Cat#: LVP304-PBS