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商品編號


PB-MIR302



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


piggybac transposon



商品信息

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Overview:
Easy transgenesis means easy reprogramming

Easily and efficiently deliver the miR-302/367 cluster for iPSC generation using the PB-CMV-miR-302/367-EF1α-GFP iPSC PiggyBac Vector. This pre-built vector is ready-to-co-transfect with the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1), and expresses miR-302a, miR-302b, miR-302c, miR-302d, and miR-306 with the strong CMV promoter. The vector also includes a GFP reporter to simplify selection of transfectants.



With the PiggyBac Transposon System, you can:


Make transgenic cell lines with a single transfection

Integrate multiple PiggyBac Vectors in a single transfection

Insert an expression cassette into human, mouse, and rat cells

Deliver virtually any-sized DNA insert, from 10 – 100 kb

Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or induc
IBL
e promoters and include a variety of
Marker
s

Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)


Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:


Academic Customers—End-User License Information

Commercial Customers—End-User License Information


*
SBI
is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.
How It Works:
The PiggyBac Transposon System’s Cut-and-Paste Mechanism


The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.



Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism
.
The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA. The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites. The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal
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BD
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