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SBI/PB-CMV-miR-302/367-EF1α-GFP iPSC Vector/PB-MIR302/10 ?g

基因編輯
SBI/PB-CMV-miR-302/367-EF1α-GFP iPSC Vector/PB-MIR302/10 ?g


商品編號


PB-MIR302



品牌


SBI



公司


System Biosciences(SBI)



公司分類(lèi)


piggybac transposon



商品信息

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Overview:
Easy transgenesis means easy reprogramming

Easily and efficiently deliver the miR-302/367 cluster for iPSC generation using the PB-CMV-miR-302/367-EF1α-GFP iPSC PiggyBac Vector. This pre-built vector is ready-to-co-transfect with the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1), and expresses miR-302a, miR-302b, miR-302c, miR-302d, and miR-306 with the strong CMV promoter. The vector also includes a GFP reporter to simplify selection of transfectants.



With the PiggyBac Transposon System, you can:


Make transgenic cell lines with a single transfection

Integrate multiple PiggyBac Vectors in a single transfection

Insert an expression cassette into human, mouse, and rat cells

Deliver virtually any-sized DNA insert, from 10 – 100 kb

Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or induc
IBL
e promoters and include a variety of
Marker
s

Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)


Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:


Academic Customers—End-User License Information

Commercial Customers—End-User License Information


*
SBI
is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.
How It Works:
The PiggyBac Transposon System’s Cut-and-Paste Mechanism


The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.



Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism
.
The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA. The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites. The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal
Citations:
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IBL
e promoter and Cre-loxP recombination in avian cells.
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Hepatology
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Yamazaki, T, et al. (2017) Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase.
PLoS ONE
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Structure
. 2017 Jun 6; 25(6):846-857.e4. PM ID: 28479060
紀?定明香, . (2017) HTLV-1 bZIP factor は抑制性免疫補助受容體の機能を阻害し T 細胞の増殖を促進(jìn)する.
Thesis
. ;. Link: Thesis
Shinmura, K, et al. (2017) WDR62 overexpression is associated with a poor prognosis in patients with lung adenocarcinoma.
Mol. Carcinog.
. 2017 Aug 1; 56(8):1984-1991. PM ID: 28277612
Wang, L, et al. (2017) Derivation and characterization of primordial germ cells from Guangxi yellow-feather chickens..
Poult. Sci.
. 2017 May 1; 96(5):1419-1425. PM ID: 28158811
Chiu, LD, et al. (2017) Protein expression guided chemical profiling of living cells by the simultaneous observation of Raman scattering and anti-Stokes fluorescence emission.
Sci Rep
. 2017 Mar 8; 7:43569. PM ID: 28272392
Shinmura, K, et al. (2017) Reduced expression of the DNA glycosylase gene MUTYH is associated with an increased number of somatic mutations via a reduction in the DNA repair capacity in prostate adenocarcinoma.
Mol. Carcinog.
. 2017 Feb 1; 56(2):781-788. PM ID: 27253753
Lin, W & Li, Z. (2017) Blueberries inhibit cyclooxygenase-1 and cyclooxygenase-2 activity in human epithelial ovarian cancer.
Oncol Lett
. 2017 Jun 1; 13(6):4897-4904. PM ID: 28599493
Sugiyama, H, et al. (2017) Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells.
Proc. Natl. Acad. Sci. U.S.A.
. 2017 Jan 10; 114(2):340-345. PM ID: 28003464
Cammareri, P, et al. (2017) TGFβ pathway limits dedifferentiation following WNT and MAPK pathway activation to suppress intestinal tumourigenesis.
Cell Death Differ.
. 2017 Jun 16;. PM ID: 28622298
Katayama, M, et al. (2017) Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions.
J. Reprod. Dev.
. 2017 Jun 21; 63(3):311-318. PM ID: 28331164
Henssen, AG, et al. (2017) PG
BD
5 promotes site-specific oncogenic mutations in human tumors.
Nat. Genet.
. 2017 Jul 1; 49(7):1005-1014. PM ID: 28504702
Kuan, II, et al. (2017) EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2α.
Sci Rep
. 2017 Feb 3; 7:41852. PM ID: 28157205
Xu, X, et al. (2017) Reversal of Phenotypic Abnormalities by CRISPR/Cas9-Mediated Gene Correction in Huntington Disease Patient-Derived Induced Pluripotent Stem?Cells..
Stem Cell Reports
. 2017 Mar 14; 8(3):619-633. PM ID: 28238795
Kinosada, H, et al. (2017) HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors..
PLoS Pathog.
. 2017 Jan 1; 13(1):e1006120. PM ID: 28046066
Wang, G, et al. (2017) Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies.
Nat Protoc
. 2017 Jan 1; 12(1):88-103. PM ID: 27929521
Mitzelfelt, KA, et al. (2017) Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with?Selection.
Stem Cell Reports
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Zamboni, CG, et al. (2017) Polymeric nanoparticles as cancer-specific DNA delivery vectors to human hepatocellular carcinoma.
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