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商品編號


EXOQ5A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome isolation


商品信息

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Overview:
A better way to isolate exosomes

“We therefore pursued the ExoQuick
?
method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models
1
.”

?
Need exosomes?
SBI
’s ExoQuick, original formulation, enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 100 ?l) of serum, plasma, or ascites fluid. Compat
IBL
e with a wide variety of downstream applications, ExoQuick is an effective and proven alternative to ultracentrifugation
1-3
.


ExoQuick’s fast, ultracentrifugation-free method:


Saves time and labor

Is easily scalable

Conserves precious sample

Delivers high yields of functional, high quality exosomes

Can be used to isolate exosomes for a wide range of downstream applications, including

Bio
Marker
studies

Exosomal miRNA profiling

Exosomal proteomics

Exosomal lipidomics/metabolomics

Functional studies, such as in cell-to-cell signaling

Basic
BIOLOG
y, such as role in tum
Origen
esis




ExoQuick is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for res
USP
ension in an appropriate solution.




Biofluid

Sample volume

ExoQuick-TC Volume





Tissue culture media, urine, cerebrospinal fluid (CSF),
etc
.

5 mL

1 mL





In electron microscopy studies, exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation
1-2
, and these exosomes are also active in numerous functional assays
1-3
.

Exosomes isolated with ExoQuick can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting,
ELISA
, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.

Exosomes isolated with ExoQuick also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compat
IBL
e with nucleic acids isolated from ExoQuick-purified exosomes.

Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.

ExoQuick exosome isolation methods are patented technologies
4
.

Choose the right ExoQuick for your biofluid:




Catalog #

Product

Biofluid





EXOQ5A-1, EXOQ20A-1

The Original ExoQuick

For serum, plasma, and ascites fluid



EXOTC10A-1, EXOTC50A-1

ExoQuick-TC
?

For all other biofluids



EXOLP5A-1

ExoQuick-LP

For removing contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation



EXOQ5TM-1

ExoQuick Plasma Prep with Thrombin

For de-fibrinating plasma before exosome isolation for efficient recovery and high yields



EXOCG50A-1

ExoQuick-CG

For preparing exosomes used in pre-clinical
in vivo
applications



EQPL10A-1, EQPL10TC

ExoQuick PLUS and ExoQuick-TC PLUS

For sensitive applications such as mass spectrometry, exosome labeling, and
in vivo
/
ex vivo
exosome delivery





Choose between ExoQuick/ExoQuick-TC and ExoQuick PLUS/ExoQuick-TC PLUS based on your application





ExoQuick
ExoQuick-TC

ExoQuick PLUS
ExoQuick-TC PLUS




Protein Detection




Western blotting for general exosome
Marker
s (
e.g
. CD9, CD63, CD81, TSG101, Alix)

?????

?????



High-sensitivity Western blotting (
e.g
. low abundance bio
Marker
s)

???

?????




qPCR Analysis




qPCR of coding and non-coding RNAs (
e.g
. mRNA, miRNA, and lncRNA)

?????

?????




High-throughput Bio
Marker
Discovery




RNA-seq of exosomal RNAs

?????

?????



Mass spectrometry of exosomal proteins

???

?????



Lipidomics/metabolomics of exosomal cargo

???

?????




Exosome Labeling


???

?????



In vivo/ex vivo Exosome Delivery

???

?????




?????
Highly recommended,
?
Not recommended






REFERENCES


Chugh PE,
et al
. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies.
PLoS Pathog.
2013.
9
(7): e1003484. PMCID: PMC3715412.



Umezu T,
et al
. Leukemia cell to endothelial cell communication via exosomal miRNAs.
Oncogene
. 2013 May 30.
32
(22):2747-55. PMID: 22797057.



Sohel MM,
et al
. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence.
PLoS One
. 2013 Nov 4.
8
(11):e78505. PMCID: PMC3817212.



Antes T,
et al
. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.


How It Works:
High-throughput, quantitative exosome recovery

ExoQuick can be used to purify exosomes from plasma
1
, serum
2
, and malignant ascites
3
. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.


To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:
Add an appropriate volume of ExoQuick to as little as 100 ?l sample Incubate for at least one hour at 4°C Isolate exosomes with a 30-minute low-speed spin (1500
g
).
Isolated exosomes can be found in the pellet and res
USP
ended in an appropriate solution.


You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome
Marker
s (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.

REFERENCES
Chugh PE,
et al
. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies.
PLoS Pathog.
2013.
9
(7): e1003484. PMCID: PMC3715412. Epple LM,
et al
. Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles.
PLoS ONE
. 2012.
7
(7): e42064. PMCID: PMC3407172. As featured in: Exosome Isolation for Proteomic Analyses and RNA Profiling Douglas D. Taylor, Wolfgang Zacharias and Cicek Gercel-Taylor, Serum/Plasma Proteomics, Methods in Molecular
BIOLOG
y, 2011, Volume 728, Part 4, 235-246.
Citations:
Nocera, AL, et al. (2017) Exosomes mediate interepithelial transfer of functional P-glycoprotein in chronic rhinosinusitis with nasal polyps.
Laryngoscope
. 2017 May 9;. PM ID: 28485529
Barteneva, NS, et al. (2017) Extracellular vesicles in gastrointestinal cancer in conjunction with microbiota: On the border of Kingdoms.
Biochim. Biophys. Acta
. 2017 Jun 29;. PM ID: 28669749
McCommis, KS, et al. (2017) Targeting the mitochondrial pyruvate carrier attenuates fibrosis in a mouse model of nonalcoholic steatohepatitis.
Hepatology
. 2017 May 1; 65(5):1543-1556. PM ID: 28027586
Oliveira-Rodríguez, M, et al. (2017) Point-of-care detection of extracellular vesicles: Sensitivity optimization and multiple-target detection.
Biosens Bioelectron
. 2017 Jan 15; 87:38-45. PM ID: 27517736
Street, JM, et al. (2017) Urine Exosomes: An Emerging Trove of Bio
Marker
s..
Adv Clin Chem
. 2017 Jan 6; 78:103-122. PM ID: 28057185
Yap, T, et al. (2017) Oral swirl samples – a robust source of microRNA protected by extracellular vesicles..
Oral Dis
. 2017 Apr 1; 23(3):312-317. PM ID: 27796067
CUI, C, et al. (2017) 結直腸癌患者
血清
外泌體代替腫瘤組織檢測 K-Ras
基因突變
的研究.
萬(wàn)方數據資源系統
. ; 22(5). Link: 萬(wàn)方數據資源系統
Lannigan, J & Erdbruegger, U. (2017) Imaging flow cytometry for the characterization of extracellular vesicles..
Methods
. 2017 Jan 1; 112:55-67. PM ID: 27721015
Elshelmani, H & Rani, S. (2017) Exosomal MicroRNA Discovery in Age-Related Macular Degeneration.
Methods Mol. Biol.
. 2017 Nov 9; 1509:93-113. PM ID: 27826921
Mullins, RJ, et al. (2017) Exosomal bio
Marker
s of brain insulin resistance associated with regional atrophy in Alzheimer’s disease.
Hum Brain Mapp
. 2017 Apr 1; 38(4):1933-1940. PM ID: 28105773
Qi, H, et al. (2017) Using endogenous ligands for direct superparamagnetic nanoparticle cluster-based body fluid exosome separation.
RSC Advances
. 2016 Nov 27; 7:2926-2933. Link: RSC Advances
Takeshita, F. (2017) MicroRNAs: Emerging Novel Targets of Cancer Therapies.
Book
. ;. Link: Book
Cheng, R, et al. (2017) SAT-122-Integrated analysis of exosomal microRNA, gene, and pathway regulatory networks in fibrosis and hepatocarcinogenesis.
Journal of Hepatology
. ; 66(1):S638. Link: Journal of Hepatology
Cho, YE, et al. (2017) Increased liver-specific proteins in circulating extracellular vesicles as potential bio
Marker
s for drug- and alcohol-induced liver injury.
PLoS ONE
. 2017 Feb 22; 12(2):e0172463. PM ID: 28225807
Wu, Q, et al. (2017) Suppression of endothelial cell migration by tumor associated macrophage-derived exosomes is reversed by epithelial ovarian cancer exosomal lncRNA.
Cancer Cell Int.
. 2017 Jun 8; 17:62. PM ID: 28592924
Cho, YE, et al. (2017) Circulating Plasma and Exosomal microRNAs as Indicators of Drug-Induced Organ Injury in Rodent Models.
Biomol Ther (Seoul)
. 2017 Jul 1; 25(4):367-373. PM ID: 28208010
Russo, F, et al. (2017) Circulating Noncoding RNAs as Clinical Bio
Marker
s.
Book
. ;. Link: Book
Huang, Z, et al. (2017) A novel serum microRNA signature to screen esophageal squamous cell carcinoma.
Cancer Med
. 2017 Jan 1; 6(1):109-119. PM ID: 28035762
B Moyron, R, et al. (2017) Differential protein expression in exosomal samples taken from trauma patients.
Proteomics Clin Appl
. 2017 May 25;. PM ID: 28544811
Huang-Doran, I, Zhang, CY & Vidal-Puig, A. (2017) Extracellular Vesicles: Novel Mediators of Cell Communication In Metabolic Disease..
Trends Endocrinol. Metab.
. 2017 Jan 1; 28(1):3-18. PM ID: 27810172