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商品編號


EQPL10A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome isolation



商品信息

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Overview:
Quick and efficient exosome isolation for sensitive applications

While the ExoQuick
?
family of reagents speed-up and simplify exosome isolation and are compat
IBL
e with most downstream uses, some applications are especially sensitive to carry-over of non-exosomal proteins—applications such as mass spectrometry, Western blotting of low abundance
Marker
s, exosome labeling, and
in vivo?
/
ex vivo
exosome delivery. Which is why
SBI
offers ExoQuick PLUS.


Less is more—ExoQuick PLUS and ExoQuick-TC PLUS for when you need an extra level of confidence:


Better—delivers as much as 150-fold higher yields than ultracentrifugation-based methods

Cleaner—dramatically reduces the amount of carry-over protein compared to other column- or precipitation-based kits, and even ultracentrifugation-based methods

Powerful—detects bio
Marker
s with as much as a 3-fold increase in sensitivity

Fast—takes less than 10 minutes of hands-on-time after initial ExoQuick isolation


Building on our popular ExoQuick family of products, ExoQuick PLUS delivers dramatically reduced albumin contamination in serum/plasma preparations compared to utracentrifugation-based isolation methods (Supporting Data, Figure 1), and considerably less carryover protein compared to other exosome isolation kits (Supporting Data, Figure 2). Even better, isolation with the ExoQuick PLUS family of products results in much higher yields than isolation with ultracentrifugation—as much as 150-fold higher (Supporting Data, Figure 3).

With a simple treatment of our ready-to-use, customized ExoQuick PLUS Microspheres (kits contain enough ExoQuick and pre-packed Microspheres for 10 reactions*), you can obtain highly pure, intact exosomes suitable for a variety of protein-sensitive applications. Total hands-on time after ExoQuick isolation is less than 10 minutes!

*For exosome isolation from serum or plasma, one reaction is defined as 250 ?L of serum or plasma. For exosome isolation from tissue culture media, one reaction is defined as 5 mL of media.


Learn more about ExoQuick—click here.

Choose the right ExoQuick for your biofluid:




Catalog #

Product

Biofluid





EXOQ5A-1, EXOQ20A-1

The Original ExoQuick

For serum, plasma, and ascites fluid



EXOTC10A-1, EXOTC50A-1

ExoQuick-TC
?

For all other biofluids



EXOLP5A-1

ExoQuick-LP

For removing contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation



EXOQ5TM-1

ExoQuick Plasma Prep with Thrombin

For de-fibrinating plasma before exosome isolation for efficient recovery and high yields



EXOCG50A-1

ExoQuick-CG

For preparing exosomes used in pre-clinical
in vivo
applications



EQPL10A-1, EQPL10TC

ExoQuick PLUS and ExoQuick-TC PLUS

For sensitive applications such as mass spectrometry, exosome labeling, and
in vivo
/
ex vivo
exosome delivery





Choose between ExoQuick/ExoQuick-TC and ExoQuick PLUS/ExoQuick-TC PLUS based on your application





ExoQuick
ExoQuick-TC

ExoQuick PLUS
ExoQuick-TC PLUS




Protein Detection




Western blotting for general exosome
Marker
s (
e.g
. CD9, CD63, CD81, TSG101, Alix)

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?????



High-sensitivity Western blotting (
e.g
. low abundance bio
Marker
s)

???

?????




qPCR Analysis




qPCR of coding and non-coding RNAs (
e.g
. mRNA, miRNA, and lncRNA)

?????

?????




High-throughput Bio
Marker
Discovery




RNA-seq of exosomal RNAs

?????

?????



Mass spectrometry of exosomal proteins

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?????



Lipidomics/metabolomics of exosomal cargo

???

?????




Exosome Labeling


???

?????



In vivo/ex vivo Exosome Delivery

???

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Highly recommended,
?
Not recommended





How It Works:
High-throughput, quantitative exosome recovery

ExoQuick PLUS can be used to purify exosomes from plasma
1
, serum
2
, and malignant ascites
3
. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick PLUS is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples that will then be used in sensitive downstream applications.


To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:
Add an appropriate volume of ExoQuick PLUS to as little as 100 ?l sample Incubate for at least one hour at 4°C Pellet exosomes with a 30-minute low-speed spin (1500
g
) Res
USP
end pellet, add ExoQuick PLUS Microspheres, and incubate for 10 minutes at 4°C Remove microsopheres with a 30-minute low-speed spin (1500g)
ExoQuick PLUS-isolated exosomes can be found in the supernatant.


You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome
Marker
s (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

REFERENCES
Chugh PE,
et al
. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies.
PLoS Pathog.
2013.
9
(7): e1003484. PMCID: PMC3715412. Epple LM,
et al
. Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles.
PLoS ONE
. 2012.
7
(7): e42064. PMCID: PMC3407172. As featured in: Exosome Isolation for Proteomic Analyses and RNA Profiling Douglas D. Taylor, Wolfgang Zacharias and Cicek Gercel-Taylor, Serum/Plasma Proteomics, Methods in Molecular
BIOLOG
y, 2011, Volume 728, Part 4, 235-246.