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商品編號


EXOQ5TM-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome isolation


商品信息

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Overview:
Efficient exosome isolation from plasma

“We therefore pursued the ExoQuick
?
method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models
1
.”

?
When isolating exosomes from plasma, fibrinogen and fibrin can impede efficient recovery. By pre-treating plasma with thrombin, the fibrinogen can be converted to fibrin and easily pre-cleared by a simple centrifugation step. The resulting serum-like solution can now be treated with ExoQuick, original formulation, for quantitative exosome isolation that is compat
IBL
e with high-throughput methods.

The ExoQuick Plasma Prep with Thrombin Kit includes both ExoQuick and thrombin for efficient exosome isolation from plasma.


ExoQuick’s fast, ultracentrifugation-free method:


Saves time and labor

Is easily scalable

Conserves precious sample

Delivers high yields of functional, high quality exosomes

Can be used to isolate exosomes for a wide range of downstream applications, including

Bio
Marker
studies

Exosomal miRNA profiling

Exosomal proteomics

Exosomal lipidomics/metabolomics

Functional studies, such as in cell-to-cell signaling

Basic
BIOLOG
y, such as role in tum
Origen
esis




ExoQuick is a proprietary polymer that gently precipitates exosomes. For exosome isolation from plasma you can first defibrinate your sample with thrombin, and then continue with the standard ExoQuick protocol of pre-clearing your sample of cells and cellular debris, adding the appropriate amount of ExoQuick, refrigerating, and then centrifuging (see the product manual for protocol details). Your exosomes will be in the pellet, ready for res
USP
ension in an appropriate solution.




Biofluid

Sample volume

ExoQuick-TC Volume





Tissue culture media, urine, cerebrospinal fluid (CSF),
etc
.

5 mL

1 mL




In electron microscopy studies, exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation
1-2
, and these exosomes are also active in numerous functional assays
1-3
.

Exosomes isolated with ExoQuick can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting,
ELISA
, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.

Exosomes isolated with ExoQuick also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compat
IBL
e with nucleic acids isolated from ExoQuick-purified exosomes.

Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.

ExoQuick exosome isolation methods are patented technologies
4
.

Choose the right ExoQuick for your biofluid:




Catalog #

Product

Biofluid





EXOQ5A-1, EXOQ20A-1

The Original ExoQuick

For serum, plasma, and ascites fluid



EXOTC10A-1, EXOTC50A-1

ExoQuick-TC
?

For all other biofluids



EXOLP5A-1

ExoQuick-LP

For removing contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation



EXOQ5TM-1

ExoQuick Plasma Prep with Thrombin

For de-fibrinating plasma before exosome isolation for efficient recovery and high yields



EXOCG50A-1

ExoQuick-CG

For preparing exosomes used in pre-clinical
in vivo
applications



EQPL10A-1, EQPL10TC

ExoQuick PLUS and ExoQuick-TC PLUS

For sensitive applications such as mass spectrometry, exosome labeling, and
in vivo
/
ex vivo
exosome delivery





REFERENCES


Chugh PE,
et al
. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies.
PLoS Pathog.
2013.
9
(7): e1003484. PMCID: PMC3715412.



Umezu T,
et al
. Leukemia cell to endothelial cell communication via exosomal miRNAs.
Oncogene
. 2013 May 30.
32
(22):2747-55. PMID: 22797057.



Sohel MM,
et al
. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence.
PLoS One
. 2013 Nov 4.
8
(11):e78505. PMCID: PMC3817212.



Antes T,
et al
. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.


How It Works:
High-throughput, quantitative exosome recovery from plasma

ExoQuick Plasma Prep with Thrombin can be used to purify exosomes from plasma
1
. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.


To isolate exosomes from plasma simply:
Incubate with thrombin to convert fibrinogen to fibrin Clear fibrin, cells, and cell debris with a quick low-speed spin Add an appropriate volume of ExoQuick to as little as 100 ?l sample Incubate for at least one hour at 4°C Isolate exosomes with a 30-minute low-speed spin (1500
g
).
Isolated exosomes can be found in the pellet and res
USP
ended in an appropriate solution.


You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome
Marker
s (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.

REFERENCES
Chugh PE,
et al
. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies.
PLoS Pathog.
2013.
9
(7): e1003484. PMCID: PMC3715412.