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商品編號


EXOTC10A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome isolation


商品信息

.img-style{heigth:450px;width:450px;}
Overview:
A better way to isolate exosomes

“We therefore pursued the ExoQuick
?
method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models
1
.”

?
Need exosomes?
SBI
’s ExoQuick-TC enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 1 ml) of most biofluids (for serum, plasma, or ascites fluid, use the original ExoQuick formulation (EXOQ5A-1 or EXOQ20A-1)). Compat
IBL
e with a wide variety of downstream applications, ExoQuick-TC is an effective and proven alternative to ultracentrifugation
1-3
.


ExoQuick-TC’s fast, ultracentrifugation-free method:


Saves time and labor

Is easily scalable

Conserves precious sample

Delivers high yields of functional, high quality exosomes

Can be used to isolate exosomes for a wide range of downstream applications, including

Bio
Marker
studies

Exosomal miRNA profiling

Exosomal proteomics

Exosomal lipidomics/metabolomics

Functional studies, such as in cell-to-cell signaling

Basic
BIOLOG
y, such as role in tum
Origen
esis




ExoQuick-TC is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick-TC to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for res
USP
ension in an appropriate solution.




Biofluid

Sample volume

ExoQuick-TC Volume





Tissue culture media, urine, cerebrospinal fluid (CSF),
etc
.

5 mL

1 mL





In electron microscopy studies, exosomes isolated with ExoQuick-TC appear similar to exosomes isolated using ultracentrifugation
1-2
, and these exosomes are also active in numerous functional assays
1-3
.

Exosomes isolated with ExoQuick-TC can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting,
ELISA
, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.

Exosomes isolated with ExoQuick-TC also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compat
IBL
e with nucleic acids isolated from ExoQuick-TC-purified exosomes.

Backed by a growing number of publications, ExoQuick-TC is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.

ExoQuick-TC exosome isolation methods are patented technologies
4
.

Choose the right ExoQuick for your biofluid:




Catalog #

Product

Biofluid





EXOQ5A-1, EXOQ20A-1

The Original ExoQuick

For serum, plasma, and ascites fluid



EXOTC10A-1, EXOTC50A-1

ExoQuick-TC
?

For all other biofluids



EXOLP5A-1

ExoQuick-LP

For removing contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation



EXOQ5TM-1

ExoQuick Plasma Prep with Thrombin

For de-fibrinating plasma before exosome isolation for efficient recovery and high yields



EXOCG50A-1

ExoQuick-CG

For preparing exosomes used in pre-clinical
in vivo
applications



EQPL10A-1, EQPL10TC

ExoQuick PLUS and ExoQuick-TC PLUS

For sensitive applications such as mass spectrometry, exosome labeling, and
in vivo
/
ex vivo
exosome delivery





Choose between ExoQuick/ExoQuick-TC and ExoQuick PLUS/ExoQuick-TC PLUS based on your application





ExoQuick
ExoQuick-TC

ExoQuick PLUS
ExoQuick-TC PLUS




Protein Detection




Western blotting for general exosome
Marker
s (
e.g
. CD9, CD63, CD81, TSG101, Alix)

?????

?????



High-sensitivity Western blotting (
e.g
. low abundance bio
Marker
s)

???

?????




qPCR Analysis




qPCR of coding and non-coding RNAs (
e.g
. mRNA, miRNA, and lncRNA)

?????

?????




High-throughput Bio
Marker
Discovery




RNA-seq of exosomal RNAs

?????

?????



Mass spectrometry of exosomal proteins

???

?????



Lipidomics/metabolomics of exosomal cargo

???

?????




Exosome Labeling


???

?????



In vivo/ex vivo Exosome Delivery

???

?????




?????
Highly recommended,
?
Not recommended






REFERENCES


Chugh PE,
et al
. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies.
PLoS Pathog.
2013.
9
(7): e1003484. PMCID: PMC3715412.



Umezu T,
et al
. Leukemia cell to endothelial cell communication via exosomal miRNAs.
Oncogene
. 2013 May 30.
32
(22):2747-55. PMID: 22797057.



Sohel MM,
et al
. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence.
PLoS One
. 2013 Nov 4.
8
(11):e78505. PMCID: PMC3817212.



Antes T,
et al
. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.


How It Works:
High-throughput, quantitative exosome recovery

ExoQuick-TC can be used to purify exosomes from a wide variety of biofluids, including saliva
1
, urine
2
, follicular fluid
3
, tissue culture media
4
, and breast milk
5
. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-TC is an excellent option for researchers who need to purify multiple exosome samples.


To isolate exosomes from any cleared biofluid, simply:
Add an appropriate volume of ExoQuick-TC Incubate for at least one hour at 4°C Isolate exosomes with a 30-minute low-speed spin (1500
g
).
Isolated exosomes can be found in the pellet and res
USP
ended in an appropriate solution.


You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome
Marker
s (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick-TC, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.

REFERENCES
Yang J,
et al
. Detection of Tumor Cell-Specific mRNA and Protein in Exosome-Like Microvesicles from Blood and Saliva.
PLoS ONE.
2014.
9
(11): e110641. PMCID: PMC 4232306. Alvarez ML. Isolation of urinary exosomes for RNA bio
Marker
discovery using a simple, fast, and highly scalable method.
Methods Mol Biol
. 2014.
1182
:145-70. PMID: 25055908. Sohel MM,
et al
. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence.
PLoS One
. 2013 Nov 4.
8
(11):e78505. PMCID: PMC3817212. Zhu L,
et al
. Novel method for extracting exosomes of hepatocellular carcinoma cells. World
J Gastroenterol
. 2014 June 7.
20
(21): 6651-6657. PMCID: PMC4047354. Gu Y,
et al
. Lactation-Related MicroRNA Expression Profiles of Porcine Breast Milk Exosomes.
PLoS ONE
. 2012.
7
(8): e43691. PMCID: PMC3427246.
Citations:
Jiang, S, et al. (2017) Role of microRNA-130b in placental PGC-1α/TFAM mitochondrial biogenesis pathway.
Biochem. Biophys. Res. Commun.
. 2017 Jun 3; 487(3):607-612. PM ID: 28433632
Galazka, G, et al. (2017) Multiple sclerosis: Serum-derived exosomes express myelin proteins.
Mult. Scler.
. 2017 Feb 1;:1352458517696597. PM ID: 28273783
Gholizadeh, S, et al. (2017) Microfluidic approaches for isolation, detection, and characterization of extracellular vesicles: Current status and future directions.
Biosens Bioelectron
. 2017 May 15; 91:588-605. PM ID: 28088752
Szatmári, T, et al. (2017) Extracellular Vesicles Mediate R
ADI
ation-Induced Systemic Bystander Signals in the Bone Marrow and Spleen.
Front Immunol
. 2017 Apr 11; 8:347. PM ID: 28396668
Lim, JH, et al. (2017) Comparative analysis of microRNA and mRNA expression profiles in cells and exosomes under toluene exposure.
Toxicol In Vitro
. 2017 Jun 1; 41:92-101. PM ID: 28245982
Slowey, PD. (2017) Salivary Diagnostics Using Purified Nucleic Acids.
Methods Mol. Biol.
. 2017 Dec 7; 1537:3-15. PM ID: 27924585
Fry, CS, et al. (2017) Myogenic
Progen
itor Cells Control Extracellular Matrix Production by Fibroblasts during Skeletal Muscle Hypertrophy.
Cell Stem Cell
. 2017 Jan 5; 20(1):56-69. PM ID: 27840022
Chen, L, et al. (2017) Exosomes derived from human menstrual blood-derived stem cells alleviate fulminant hepatic failure.
Stem Cell Res Ther
. 2017 Jan 23; 8(1):9. PM ID: 28115012
Qu, Y, et al. (2017) Exosomes derived from miR-181-5p-modified
ADI
pose-derived mesenchymal stem cells prevent liver fibrosis via autophagy activation.
J. Cell. Mol. Med.
. 2017 Apr 6;. PM ID: 2838
2720
Maeda, Y, et al. (2017) Synovium-Derived MicroRNAs Regulate Bone Pathways in Rheumatoid Arthritis.
J. Bone Miner. Res.
. 2017 Mar 1; 32(3):461-472. PM ID: 27676131
Umezu, T, et al. (2017) Replenishing exosomes from older bone marrow stromal cells with miR-340 inhibits myeloma-related angiogenesis.
Blood Advances
. 2017 May 16; 1(13):812-823. Link: Blood Advances
Saha, B, et al. (2017) Circulating and Exosome-Packaged Hepatitis C Single-Stranded RNA Induce Monocyte Differentiation via TLR7/8 to Polarized Macrophages and Fibrocytes.
J. Immunol.
. 2017 Mar 1; 198(5):1974-1984. PM ID: 28122964
Hoptay, CE, Ferrante, S & Hackett, TL. (2017) Asthmatic Bronchial Airway Epithelial Exosomes Do Not Promote Mesenchymal Fibrosis Despite Exosomal MicroRNA Predicted Targets.
Conference
. ;. Link: Conference
Huang, Z, et al. (2017) Urinary Exosomal miR-193a Can Be a Potential Bio
Marker
for the Diagnosis of Primary Focal Segmental Glomerulosclerosis in Children.
Biomed Res Int
. 2017 Mar 1; 2017:7298160. PM ID: 28246603
Vanni, I, et al. (2017) Exosomes: a new horizon in lung cancer.
Drug Discov. Today
. 2017 Jun 1; 22(6):927-936. PM ID: 28288782
Bomfim, MM. (2017) Efeitos do estresse oxidativo durante a produ??o in vitro de embri?es bovinos sobre o miR-199a e genes alvo ERBB2 e ERBB3.
Thesis
. ;. Link: Thesis
Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology.
Pediatr. Res.
. 2017 Apr 5;. PM ID: 28157838
Kim, MS. (2017) Exosome-Encapsulated Water-Insoluble Small Molecule Chemotherapeutics for the Treatment of Pulmonary Metastases.
Poster
. ;. Link: Poster
Nuzhat, Z, et al. (2017) Tumour-derived exosomes as a signature of pancreatic cancer – liquid biopsies as indicators of tumour progression.
Oncotarget
. 2017 Mar 7; 8(10):17279-17291. PM ID: 27999198
Osawa, S, et al. (2017) Fibronectin on extracellular vesicles from microvascular endothelial cells is involved in the vesicle uptake into oligodendrocyte precursor cells.
Biochem. Biophys. Res. Commun.
. 2017 Jun 17; 488(1):232-238. PM ID: 28499870