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商品編號


EXOCG50A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome isolation


商品信息

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Overview:
ExoQuick-CG: cGMP quality exosome isolation for pre-clinical applications

“We therefore pursued the ExoQuick
?
method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models
1
.”

?
Based on
SBI
’s well-regarded ExoQuick-TC,
SBI
’s ExoQuick-CG exosome precipitation reagent makes miRNA and protein bio
Marker
discoveries simple, reliable, and quantitative. Enrich for exosomal miRNAs with ExoQuick-CG and accurately profile them using
SBI
’s SeraMir qPCR arrays. Downstream protein analysis is also poss
IBL
e with
SBI
’s exosome specific antibodies and
ELISA
kits.


Enjoy the same benefits of ExoQuick-TC as well as these additional benefits:


21 CFR 820 compliant

All ingredients sourced from GMP manufacturer

Reagent mixed under cGMP conditions at GMP facility

Triple filtered

Sterility tested

Works in all biofluids tested


Like ExoQuick-TC, ExoQuick-CG enables high-throughput, quantitative isolation of exosomes from most biofluids. Compat
IBL
e with a wide variety of downstream applications, ExoQuick-CG is an effective and proven alternative to ultracentrifugation
1-3
.

Choose the right ExoQuick for your biofluid:




Catalog #

Product

Biofluid





EXOQ5A-1, EXOQ20A-1

The Original ExoQuick

For serum, plasma, and ascites fluid



EXOTC10A-1, EXOTC50A-1

ExoQuick-TC
?

For all other biofluids



EXOLP5A-1

ExoQuick-LP

For removing contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation



EXOQ5TM-1

ExoQuick Plasma Prep with Thrombin

For de-fibrinating plasma before exosome isolation for efficient recovery and high yields



EXOCG50A-1

ExoQuick-CG

For preparing exosomes used in pre-clinical
in vivo
applications



EQPL10A-1, EQPL10TC

ExoQuick PLUS and ExoQuick-TC PLUS

For sensitive applications such as mass spectrometry, exosome labeling, and
in vivo
/
ex vivo
exosome delivery





REFERENCES


Chugh PE,
et al
. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies.
PLoS Pathog.
2013.
9
(7): e1003484. PMCID: PMC3715412.



Umezu T,
et al
. Leukemia cell to endothelial cell communication via exosomal miRNAs.
Oncogene
. 2013 May 30.
32
(22):2747-55. PMID: 22797057.



Sohel MM,
et al
. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence.
PLoS One
. 2013 Nov 4.
8
(11):e78505. PMCID: PMC3817212.


How It Works:
High-throughput, quantitative exosome recovery

Just as with ExoQuick-TC, ExoQuick-CG can be used to purify exosomes from saliva
1
, urine
2
, follicular fluid
3
, tissue culture media
4
, and breast milk
5
. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-CG is an excellent option for researchers who need to purify exosomes for pre-clinical,
in vivo
studies.


To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:
Add an appropriate volume of ExoQuick-CG Incubate for at least one hour at 4°C Isolate exosomes with a 30-minute low-speed spin (1500
g
).
Isolated exosomes can be found in the pellet and res
USP
ended in an appropriate solution.


You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome
Marker
s (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.

REFERENCES
Yang J,
et al
. Detection of Tumor Cell-Specific mRNA and Protein in Exosome-Like Microvesicles from Blood and Saliva.
PLoS ONE.
2014.
9
(11): e110641. PMCID: PMC 4232306. Alvarez ML. Isolation of urinary exosomes for RNA bio
Marker
discovery using a simple, fast, and highly scalable method.
Methods Mol Biol
. 2014.
1182
:145-70. PMID: 25055908. Sohel MM,
et al
. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence.
PLoS One
. 2013 Nov 4.
8
(11):e78505. PMCID: PMC3817212. Zhu L,
et al
. Novel method for extracting exosomes of hepatocellular carcinoma cells. World
J Gastroenterol
. 2014 June 7.
20
(21): 6651-6657. PMCID: PMC4047354. Gu Y,
et al
. Lactation-Related MicroRNA Expression Profiles of Porcine Breast Milk Exosomes.
PLoS ONE
. 2012.
7
(8): e43691. PMCID: PMC3427246.