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商品編號


EXOFLOW300A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome isolation



商品信息

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Overview:
Go with the flow for easy exosome isolation using FACS

The CD63 Exo-Flow Capture Kit has all the reagents you need to purify exosomes using FACS—magnetic streptavidin beads, CD63-biotin capture antibody, wash and elution buffers, and revers
IBL
e Exo-FITC stain (Cat.# EXOFLOW800A-1)—you supply the FACS and the optional magnetic stand (Cat.# EXOFLOW700A-1). Our well-validated CD63-biotin antibody and high-quality kit components ensure reliable, reproduc
IBL
e exosome purification based on the presence of CD63 on the exosome surface. And with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations.


Note that we also offer revers
IBL
e Exo-APC (Cat.# Exo-FLOW810A-1) as an alternative stain to Exo-FITC.


A range of well-validated antibodies for reliable and reproduc
IBL
e purification

Large-sized magnetic beads increase the efficiency of exosome capture

Revers
IBL
e Exo-FITC and Exo-APC stains can be completely removed after FACS

Exosome Elution Buffer simultaneously removes Exo-FITC (or Exo-APC) and elutes exosomes from the magnetic beads for downstream applications such as functional studies



More than just CD63s

To facilitate the widest range of studies,
SBI
built the Exo-Flow system to be highly modular. We offer a range of biotinylated antibodies as well as a Basic Exo-Flow Kit (Cat.# CSFLOWBASICA-1) with no antibody so that you can use your own biotinylated antibodies.




Cat.#

Kit





EXOFLOW100A-1

CD9 Exo-Flow Capture Kit



EXOFLOW150A-1

Tetraspanin Exo-Flow Combo Capture Kit



EXOFLOW200A-1

CD31 Exo-Flow Capture Kit



EXOFLOW210A-1

CD44 Exo-Flow Capture Kit



EXOFLOW300A-1

CD63 Exo-Flow Capture Kit



EXOFLOW400A-1

CD81 Exo-Flow Capture Kit



EXOFLOW500A-1

Rab5b Exo-Flow Capture Kit



EXOFLOW600A-1

HLA-G Exo-Flow Capture Kit



EXOFLOW610A-1

CD14 Exo-Flow Capture Kit



EXOFLOW620A-1

CD68 Exo-Flow Capture Kit



EXOFLOW630A-1

EpCAM Exo-Flow Capture Kit



EXOFLOW650A-1

α2-Macroglobulin (A2M) Exo-Flow Capture Kit



EXOFLOW660A-1

CD73 Exo-Flow Capture Kit




How It Works:
Easily purify exosomes using FACS with the CD63 Exo-Flow Capture Kit

At a glance
Simply (1) couple the CD63-biotin antibody to the magnetic streptavidin beads, (2) use the CD63-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick? or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with revers
IBL
e Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).


Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.

Citations:
Protocol, IIEI. (2017) List of Components.
Product
. ;. Link: Product
Etzerodt, A, et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma.
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Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis.
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AIDS
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Wen, Z, et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs.
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Expert Rev Proteomics
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Mukherjee, K, et al. (2016) Revers
IBL
e HuR-microRNA binding controls extracellular export of miR-122 and augments stress response.
EMBO Rep.
. 2016 Aug 1; 17(8):1184-203. PM ID: 27402548
Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry.
J Cancer Prev
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Nudel, K. (2016) Neisseria gonorrhoeae modulates epithelial cell responses via the induction and release of the inhibitor of apoptosis protein cIAP2 in exosomes.
Thesis
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Peterson, MF, et al. (2015) Integrated systems for exosome investigation.
Methods
. 2015 Oct 1; 87:31-45. PM ID: 25916618
Bhattarai, N, et al. (2015) Conserved Motifs within Hepatitis C Virus Envelope (E2) RNA and Protein Independently Inhibit T Cell Activation.
PLoS Pathog.
. 2015 Sep 1; 11(9):e1005183. PM ID: 26421924
Nudel, K, Massari, P & Genco, CA. (2015) Neisseria gonorrhoeae Modulates Cell Death in Human Endocervical Epithelial Cells through Export of Exosome-Associated cIAP2.
Infect. Immun.
. 2015 Sep 1; 83(9):3410-7. PM ID: 26077759
Bednarczyk, M, Biry?o, M & DAWIDOWICZ, A. (2015) Modern Geodetic Techniques in Spatial Measurement.
Thesis
. ;. Link: Thesis
Di Noto, G, et al. (2014) Immunoglobulin Free Light Chains and GAGs Mediate Multiple Myeloma Extracellular Vesicles Uptake and Secondary NfκB Nuclear Translocation.
Front Immunol
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Jia, S, et al. (2014) Emerging technologies in extracellular vesicle-based molecular diagnostics.
Expert Rev. Mol. Diagn.
. 2014 Apr 1; 14(3):307-21. PM ID: 24575799
Santana, SM, et al. (2014) Cancerous epithelial cell lines shed extracellular vesicles with a bimodal size distribution that is sensitive to glutamine inhibition.
Phys Biol
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Basu, S & Bhattacharyya, SN. (2014) Insulin-like growth factor-1 prevents miR-122 production in neighbouring cells to curtail its intercellular transfer to ensure proliferation of human hepatoma cells.
Nucleic Acids Res.
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