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商品編號


EXOAB-FLOT1-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome detection




商品信息

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Overview:
Confirm exosome isolation with confidence

When you’re ready to confirm that you’ve isolated exosomes, stick with the exosome experts at
SBI
. Our polyclonal rabbit anti-FLOT1 antibodies recognize the human, mouse, and rat forms of the protein, and are a great choice for general exosome detection, especially when used in conjunction with antibodies for other exosome
Marker
s such as the tetraspanins CD9, CD63, and CD81, as well as the heat shock protein HSP70 (if you have any questions about the best antibodies to use for your exosome project, we’re ready to help! Just email us at tech@systembio.com). And, like all of our antibodies, our anti-FLOT1 antibody and the included goat anti-rabbit HRP secondary antibodies are rigorously validated for exosome detection via Western blotting.



Rigorously validated

Hand-picked selection

Expert exosome support


Choose the right antibody for your exosome detection needs




Cat.#

Antibody to

Notes

Link to ExoCarta entry for the human protein





General exosome
Marker
s



EXOAB-CD9A-1

CD9

Tetraspanin

ExoCarta_928



EXOAB-CD63A-1

CD63

Tetraspanin

ExoCarta_967



EXOAB-CD81A-1

CD81

Tetraspanin

ExoCarta_93185



EXOAB-Hsp70A-1

HSP70


ExoCarta_3303, ExoCarta_3304, ExoCarta_3306, ExoCarta_3308, ExoCarta 3310, ExoCarta_3311



EXOAB-ALIX-1

ALIX

Programmed cell death 6 interacting protein (PDCD6IP)

ExoCarta_10015



EXOAB-ANXA5-1

ANXA5-1

Annexin A5

ExoCarta_308



EXOAB-TSG101-1

TSG101

Tumor susceptibility gene 101

ExoCarta_7251



EXOAB-FLOT1-1

FLOT-1

Flotillin-1

ExoCarta_10211



Tissue-specific exosome
Marker
s



EXOAB-CXCR4-1

CXCR4

Specific for B-cells, T-cells, and platelets

ExoCarta_7852



EXOAB-EPCAM1-1

EpCam

Specific for cancer-derived exosomes

ExoCarta_4072



EXOAB-VMTN-1

Vimentin

Specific for mesenchymal stem cells

ExoCarta_7431




Supporting Data:

See isolation data using Exo-FLOW IP Kits

For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.




Figure 1. CD63 and CD9, two exosome
Marker
s, are re
ADI
ly detected in samples purified using Exo-Flow Kits.
Approximately 1 ?g of protein was loaded per well on a 4-20% gr
ADI
ent protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein
Marker
s.



Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes
.